Semen Analysis (AUA Guidelines), Postvasectomy

CPT: 89310
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Synonyms

  • Postvasectomy Semen Analysis

Special Instructions

This test is not available at all locations and must be scheduled with the laboratory performing the test. Contact the laboratory prior to specimen collection. This test requires a freshly collected semen sample. If the sample is more than one hour old, please see Postvasectomy Sperm Evaluation, Qualitative [519020] for the presence or absence of sperm. This test does not include motility studies and can be conducted on any semen specimen that is submitted to LabCorp within 72 hours of collection.


Expected Turnaround Time

Within 1 day



Specimen Requirements


Specimen

Semen


Volume

Entire ejaculate


Minimum Volume

0.5 mL


Container

Sterile screw-cap container


Collection

See Patient Instructions for Semen Collection.


Storage Instructions

Testing must begin within 60 minutes of specimen production. In the interim, the specimen should be kept at room temperature, but it is recommended that it be kept close to the body (inside a shirt or coat) to avoid temperature extremes during transport.


Stability Requirements

Temperature

Period

Room temperature

1 hour; keep sample close to body (inside shirt or coat) to avoid temperature extremes during transport. (stability provided by manufacturer or literature reference)

Refrigerated

Unstable (stability provided by manufacturer or literature reference)

Frozen

Unstable (stability provided by manufacturer or literature reference)

Freeze/thaw cycles

Unstable (stability provided by manufacturer or literature reference)


Patient Preparation

No special preparation. The American Urological Association recommends a waiting period of 8 to 16 weeks post vasectomy as the appropriate period for the first postvasectomy semen analysis.1


Causes for Rejection

Specimens older than one hour; specimens contaminated with spermicidal material


Test Details


Use

Azoöspermia and <0.1x106 nonmotile sperm per mL (or <100,000 nonmotile sperm per mL) are reliable indicators of vasectomy success.

Incomplete ejaculation and/or incomplete collection of total ejaculate may cause false oligospermia (low sperm count) or false azoöspermia.

If >0.1x106 nonmotile sperm per mL (or >100,000 nonmotile sperm/mL) persist beyond six months after vasectomy, then trends of serial postvasectomy semen analyses and clinical judgment should be used to decide whether the vasectomy is a failure and whether repeat vasectomy should be considered.


Methodology

Macroscopic and microscopic semen examination


Footnotes

1. Sharlip ID, Belker AM, Honig S, et al, American Urological Association. Vasectomy: AUA guideline. J Urol. 2012 Dec; 188(6 Suppl):2482-2491. 23098786

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
519013 Post VAS Semen Analysis, AUA 4463-6 519016 Volume mL 3160-9
519013 Post VAS Semen Analysis, AUA 4463-6 519190 Total Immotile Sperm x10E6/mL 34657-7
519013 Post VAS Semen Analysis, AUA 4463-6 519191 Total Motile Sperm x10E6/mL 42531-4

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