Red Blood Cell (RBC) Antigen Typing: Jka/Jkb

CPT: 86905(x2)
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Expected Turnaround Time

2 - 4 days


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Specimen Requirements


Specimen

Whole blood


Volume

1 mL


Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Container

Lavender-top (EDTA) tube


Storage Instructions

Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze red cells.


Causes for Rejection

Gross hemolysis; bacterial or other contamination


Test Details


Use

Typing paternal RBCs for antigens that correspond to maternal antibody may aid in the prediction of hemolytic disease of the fetus and newborn. Red cell antigen typing may also be indicated for bone marrow, tissue, organ, or blood donors prior to donation. Testing a prenatal patient for red cell antigens is generally not indicated. The occurrence of the Jka antigen is 77% in Caucasians, 92% in Blacks, and 72% in Asians. Anti-Jka can cause mild to moderate (rare) hemolytic disease of the fetus and newborn. The occurrence of the Jkb antigen is 74% in Caucasians, 49% in Blacks, and 76% in Asians. Anti-Jkb can cause rare, mild hemolytic disease of the fetus and newborn.


Limitations

Presence of potent cold agglutinins, positive direct antiglobulin test, and (in some cases) bacteremia may interfere with test interpretation.


Methodology

Hemagglutination


References

Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.
Reid ME, Lomas-Frances CG, Olsson ML, eds. Blood Group Antigen Facts Book. 3rd ed. London, England: Academic Press; 2012.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006030 Jka/Jkb RBC Antigen Typing 93914-0 006028 Jka 1072-8
006030 Jka/Jkb RBC Antigen Typing 93914-0 006029 Jkb 1078-5

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