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For hours, walk-ins and appointments.Protein Electrophoresis, Serum With Reflex to IFE, Serum and Free κ and λ Light Chains, Serum
CPT:
84155; 84165
Synonyms
- Free Light Chains, Quantitative, Serum
- Freelite®
- Globulins, Serum
- IEP, Serum
- IFE, Serum and Protein Electrophoresis, Serum
- Immunoelectrophoresis for Myeloma Proteins
- Immunoelectrophoresis, Serum
- Immunofixation, Serum
- Kappa Free Light Chains, Quantitative, Serum
- Kappa/Lambda Free Light Chains, Quantitative, Serum
- Kappa:Lambda Free Light Chains Ratio, Quantitative, Serum
- Lambda Free Light Chains, Quantitative, Serum
- Light Chains, Free Kappa and Free Lambda, Quantitative, Serum
Test Includes
Serum protein electrophoresis with automatic reflex to serum immunofixation (IFE) at an additional charge if M-spike is present
Expected Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
For more information, please view the literature below.
Specimen Requirements
Specimen
Serum
Volume
5.5 mL
Minimum Volume
2.2 mL
Container
Red-top tube or gel-barrier tube
Collection
Sample should be allowed to clot and the serum separated as soon as possible to prevent hemolysis.
Storage Instructions
Refrigerate at 2°C to 8°C.
Patient Preparation
Overnight fasting is preferred, but not required.
Causes for Rejection
Lipemia; hemolysis; plasma instead of serum
Test Details
Use
Detect and identify monoclonal immunoglobulin gammopathies. Diagnose myeloma, macroglobulinemia of Waldenström. Immunofixation is used to evaluate monoclonal gammopathy (M protein) found on serum protein electrophoresis; evaluate amyloidosis. Has application to the evaluation of lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general. Of use in the diagnosis and characterization of immune deficient and dysgammaglobulinemic states. The NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines™) recommend the use of serum free light chain assays for use in the initial diagnostic workup of multiple myeloma and related disorders. "Use of free light chain (FLC) assay along with SPEP and SIFE yields high sensitivity while screening for MM and related plasma cell disorders. Therefore, this assay is now included as a part of the initial diagnostic workup in the NCCN Multiple Myeloma Guidelines."1
Methodology
PE: electrophoresis on agarose media; IFE: electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains; Immunoglobulins: turbidimetric quantitation; FLC: turbidimetric quantitation
Footnotes
1. NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines™) for Multiple Myeloma, Version 1.2011. Fort Washington, Pa: NCCN; 2011.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001073 | Protein, Total | g/dL | 2885-2 |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001488 | Albumin | g/dL | 2862-1 |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001489 | Alpha-1-Globulin | g/dL | 2865-4 |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001490 | Alpha-2-Globulin | g/dL | 2868-8 |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001491 | Beta Globulin | g/dL | 2871-2 |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001492 | Gamma Globulin | g/dL | 2874-6 |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001493 | M-Spike | g/dL | 33358-3 |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 011052 | Globulin, Total | g/dL | 10834-0 |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 011149 | A/G Ratio | 1759-0 | |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 011150 | Please note: | 77202-0 | |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001499 | IFE Reflex | N/A | |
| 123041 | PE (Rfx IFE+FLC), S | 24351-9 | 001498 | 11502-2 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 001496 | Immunofixation Reflex, Serum | 001776 | Immunoglobulin G, Qn, Serum | mg/dL | 2465-3 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 001496 | Immunofixation Reflex, Serum | 001784 | Immunoglobulin A, Qn, Serum | mg/dL | 2458-8 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 001496 | Immunofixation Reflex, Serum | 001792 | Immunoglobulin M, Qn, Serum | mg/dL | 2472-9 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 001496 | Immunofixation Reflex, Serum | 149519 | Immunofixation Result, Serum | 13169-8 | |
| Reflex 2 | 149532 | Immunofixation Interp.,Sr | 149532 | Immunofixation Interp.,Sr | 13169-8 | |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 123000 | IFE+Kappa/Lambda Qn S | 001776 | Immunoglobulin G, Qn, Serum | mg/dL | 2465-3 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 123000 | IFE+Kappa/Lambda Qn S | 001784 | Immunoglobulin A, Qn, Serum | mg/dL | 2458-8 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 123000 | IFE+Kappa/Lambda Qn S | 001792 | Immunoglobulin M, Qn, Serum | mg/dL | 2472-9 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 123000 | IFE+Kappa/Lambda Qn S | 149519 | Immunofixation Result, Serum | 13169-8 | |
| Reflex 2 | 149532 | Immunofixation Interp.,Sr | 149532 | Immunofixation Interp.,Sr | 13169-8 | |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 123000 | IFE+Kappa/Lambda Qn S | 121138 | Free Kappa Lt Chains,S | mg/L | 36916-5 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 123000 | IFE+Kappa/Lambda Qn S | 121139 | Free Lambda Lt Chains,S | mg/L | 33944-0 |
| Reflex Table for IFE Reflex | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 123000 | IFE+Kappa/Lambda Qn S | 121141 | Kappa/Lambda Ratio,S | 48378-4 | |
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