Immunofixation (IFE) and Protein Electrophoresis, 24-Hour Urine

CPT: 84156; 84166; 86335
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Synonyms

  • Bence Jones Protein Identification, 24-Hour Urine
  • Globulins, 24-Hour Urine
  • IFE/Protein Electrophoresis, 24-Hour Urine
  • Myeloma Proteins, 24-Hour Urine

Test Includes

Urine immunofixation; qualitative identification of light chains and urine protein electrophoresis


Special Instructions

The request form should include patient's age and clinical diagnosis.


Expected Turnaround Time

3 - 5 days


Related Information


Related Documents


Specimen Requirements


Specimen

Urine (24-hour); use test 122390 if random urine is obtained.


Volume

30-mL aliquot


Minimum Volume

11-mL aliquot (Note: This volume does not allow for repeat testing.)


Container

12-mL plastic transport tube (Labcorp No. 23597) without preservative


Collection

Collect 24-hour urine; mix well.


Storage Instructions

Refrigerated (preferred) or room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x1


Causes for Rejection

Preservative added


Test Details


Use

Useful in characterizing the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ globulin, γ globulin fragments (light chains, Bence Jones proteins). Of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström and amyloidosis. Monoclonal gammopathy (M spike) work-up.


Methodology

Urine immunofixation (IFE) on agarose gel; urine protein electrophoresis on agarose media; visual light-chain typing


Reference Interval

Normal pattern


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 013664 Protein,Total,Urine mg/dL 2888-6
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 013219 Prot,24hr calculated mg/24 hr 2889-4
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133751 Albumin, U % 13986-5
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133769 Alpha-1-Globulin, U % 13984-0
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133777 Alpha-2-Globulin, U % 13987-3
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133785 Beta Globulin, U % 13994-9
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133793 Gamma Globulin, U % 13989-9
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133794 M-Spike, % % 42484-6
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133796 M-Spike, mg/24 hr mg/24 hr 42482-0
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 013482 Immunofixation Result, Urine 13440-3
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 149533 Note: N/A
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 003724 PDF 11502-2

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