Immunofixation (IFE) and Protein Electrophoresis, 24-Hour Urine

CPT: 84156; 84166; 86335
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Test Details


  • Bence Jones Protein Identification, 24-Hour Urine
  • Globulins, 24-Hour Urine
  • IFE/Protein Electrophoresis, 24-Hour Urine
  • Myeloma Proteins, 24-Hour Urine

Test Includes

Urine immunofixation; qualitative identification of light chains and urine protein electrophoresis


Useful in characterizing the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ globulin, γ globulin fragments (light chains, Bence Jones proteins). Of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström and amyloidosis. Monoclonal gammopathy (M spike) work-up.


Urine immunofixation (IFE) on agarose gel; urine protein electrophoresis on agarose media; visual light-chain typing

Reference Interval

Normal pattern

Specimen Requirements


Urine (24-hour); use test 122390 if random urine is obtained.


30-mL aliquot

Minimum Volume

11-mL aliquot


12-mL plastic transport tube (LabCorp N° 23597) without preservative


Collect 24-hour urine; mix well.

Storage Instructions


Causes for Rejection

Preservative added

Clinical Information

Special Instructions

The request form should include patient's age and clinical diagnosis.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
003467 IFE+Protein Electro, 24-Hr Ur 013664 Protein,Total,Urine mg/dL 2888-6
003467 IFE+Protein Electro, 24-Hr Ur 013219 Prot,24hr calculated mg/24 hr 2889-4
003467 IFE+Protein Electro, 24-Hr Ur 133751 Albumin, U % 13986-5
003467 IFE+Protein Electro, 24-Hr Ur 133769 Alpha-1-Globulin, U % 13984-0
003467 IFE+Protein Electro, 24-Hr Ur 133777 Alpha-2-Globulin, U % 13987-3
003467 IFE+Protein Electro, 24-Hr Ur 133785 Beta Globulin, U % 13994-9
003467 IFE+Protein Electro, 24-Hr Ur 133793 Gamma Globulin, U % 13989-9
003467 IFE+Protein Electro, 24-Hr Ur 133794 M-Spike, % % 42484-6
003467 IFE+Protein Electro, 24-Hr Ur 133796 M-Spike, mg/24 hr mg/24 hr 42482-0
003467 IFE+Protein Electro, 24-Hr Ur 013482 Immunofixation Result, Urine 13440-3
003467 IFE+Protein Electro, 24-Hr Ur 149533 Note: N/A
003467 IFE+Protein Electro, 24-Hr Ur 003724 PDF 11502-2

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