Human Immunodeficiency Virus 1 (HIV-1) GenoSure PRIme®

CPT: Call 800-222-7566, ext 6-8400, for coding.
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Synonyms

  • GenoSure PRime®
  • HIV-1 Genotype
  • POL Genotype
  • Resistance Testing
  • Retroviral Genotype

Special Instructions

Submit a separate frozen specimen for each test requested.


Expected Turnaround Time

11 - 14 days



Specimen Requirements


Specimen

Plasma, frozen


Volume

5 mL


Container

Lavender-top (EDTA) or plasma preparation tubes (PPT™)


Collection

Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or Pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at - 20° C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Freeze for shipment or long-term storage.


Stability Requirements

Temperature

Period

Frozen

2 years


Causes for Rejection

PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles


Test Details


Use

Detect resistance of HIV-1 to protease, reverse transcriptase, and integrase inhibitors.


Limitations

This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and the procedure will be billed at the account's prevailing fee.


Methodology

Polymerase chain reaction (PCR) amplification and DNA sequencing.


References

Boyd SD, Maldarelli F, Sereti I, et al. Transmitted raltegravir resistance in an HIV-1 CRF_AG-infected patient. Antivir Ther. 2011; 16(2):257-261. 21447876
Panel on Antiretroviral Guidelines for Adults and Adolescents. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents. Department of Health and Human Services. Washington, DC: Department of Health and Human Services. January 28, 2000. Available at http://aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf. Accessed February 10, 2012.
Thompson MA, Aberg JA, Cahn P, et al. Antiretroviral treatment of adult HIV infection: 2010 recommendations of the International AIDS Society-USA panel. JAMA. 2010 Jul 21, 304(3):321-333. 20639566
Young B, Fransen S, Greenberg K, et al. Transmission of integrase strand-transfer inhibitor multidrug-resistant HIV-1: Case report and response to raltegravir-containing antiretroviral therapy. Antivir Ther. 2011; 16(2):253-256. 21447875

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551700 GenoSure PRIme(R) 80689-3 551703 PDF 51969-4
551700 GenoSure PRIme(R) 80689-3 551701 HIV GenoSure PRIme(R) 93047-9
551700 GenoSure PRIme(R) 80689-3 551702 HIV GenoSure PRIme(R) 80689-3
551700 GenoSure PRIme(R) 80689-3 551706 HIV GenoSure PRIme(R) Interp 62364-5
Reflex Table for HIV GenoSure PRIme(R)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551704 GenoSure PRIme(R) 551704 GenoSure PRIme(R) N/A
Reflex Table for HIV GenoSure PRIme(R)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550503 Quant, RNA PCR 550494 HIV-1 RNA by PCR copies/mL 20447-9
Reflex Table for HIV GenoSure PRIme(R)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550503 Quant, RNA PCR 550495 log10 HIV-1 RNA log10copy/mL 29541-0
Reflex Table for HIV GenoSure PRIme(R)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551707 GenoSure PRIme(R) Interp 551707 GenoSure PRIme(R) Interp N/A

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