Human Immunodeficiency Virus 1 (HIV-1) DNA Sequencing Protease−Reverse Transcriptase

CPT: Call client services.
Print Share

Synonyms

  • Cell-associated Viral DNA
  • HIV-1 Genotype
  • Resistance Analysis

Expected Turnaround Time

10 - 14 days



Specimen Requirements


Specimen

Whole blood, frozen


Volume

4 mL


Container

Lavender-top (EDTA) tube


Collection

Collect specimen in lavender-top (EDTA) tube. Do not centrifuge. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Freeze.


Causes for Rejection

Quantity not sufficient for analysis; heparinized plasma; nonfrozen specimen


Test Details


Use

Detect resistance of HIV-1 to protease and reverse transcriptase inhibitors in cell-associated viral DNA.


Limitations

This procedure should be used for patients with documented HIV-1 infection and undetectable viral load or low level viremia.


Methodology

Polymerase chain reaction (PCR) amplification and next generation DNA sequencing


References

Günthard HF, Aberg JA, Eron JJ, et al. Antiretroviral treatment of adult HIV infection: 2014 recommendations of the International Antiviral Society-USA Panel. JAMA. 2014 Jul 23-30; 312(4):410-425. 25038359
Panel on Antiretroviral Guidelines for Adults and Adolescents—A Working Group of the Office of AIDS Research Advisory Council (OARAC). Guidelines for the Use of Antiretroviral Agents in HIV-1-Infected Adults and Adolescents. Washington, DC: Department of Health and Human Services; May 1, 2014.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551730 DNA Sequencing PR-RT 80692-7 551733 PDF 80695-0
551730 DNA Sequencing PR-RT 80692-7 551731 HIV DNA Sequencing PR-RT N/A
551730 DNA Sequencing PR-RT 80692-7 551732 HIV DNA Sequencing PR-RT 80692-7
551730 DNA Sequencing PR-RT 80692-7 551729 HIV DNA Sequencing PRRT Interp 80694-3
Reflex Table for HIV DNA Sequencing PR-RT
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551734 HIVDNASeq PRRT 551734 HIVDNASeq PRRT N/A
Reflex Table for HIV DNA Sequencing PR-RT
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551743 HIVDNASeq PRRT Interp 551743 HIVDNASeq PRRT Interp N/A

For Providers

Please login to order a test

Order a Test

© 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf