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HER-2 Immunohistochemistry (IHC), Gastric With Reflex to FISH if 2+ by IHC
- HER-2/neu Oncoprotein, Paraffin Block
Evaluation of tumor tissue. Specimens demonstrating 2+ (moderate) staining for the HER-2/neu protein are evaluated for gene amplification status by FISH. In such cases, FISH provides a better indication of potential response to therapy.
Use of fixatives other than 10% formalin may not yield equivalent or satisfactory results.
Tumor (formalin-fixed, paraffin-embedded)
One paraffin block or seven unstained, positively charged tissue slides cut at 4 to 5 microns and dried for one hour at 60°C.
FFPE tissue, preserved in 10% neutral buffered formalin, fixed for 18 to 24 hours. ASCO/CAP 2013 guidelines recommend fixation time between 6 and 72 hours for breast tissue; however, special ASCO/CAP guidelines have not been established for gastric cancer samples.
Maintain specimen at room temperature.
Causes for Rejection
Tumor other than gastric tumor
Direct any questions regarding this test to oncology customer service at 800-345-4363.