Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay

CPT: 87522. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Test Details

Synonyms

  • HCV Real-time PCR With Reflex to HCV NS5A Drug Resistance Assay
  • HCV RNA Quantitation With Reflex to HCV NS5A Drug Resistance Assay

Test Includes

Reflex to HCV NS5A drug resistance assay if viral load is ≥500 IU/mL.

Use

Quantification of HCV with reflex to HCV NS5A drug resistance assay (at additional charge) if ≥500 IU/mL.

Limitations

The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.

Methodology

Cobas® AmpliPrep/Cobas® Taqman® HCV Test v 2.0. See Hepatitis C Virus (HCV) NS5A Drug Resistance Assay [550325] for more information.

Specimen Requirements

Specimen

Plasma (preferred) or serum, frozen

Volume

4 mL

Minimum Volume

2 mL

Container

Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.

Collection

Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Causes for Rejection

Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood

Clinical Information

Special Instructions

Please indicate the patient's HCV genotype (1a or 1b) on the test request form.

References

Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550349 HCV RNA PCR Qn Rfx NS5A 550073 Hepatitis C Quantitation IU/mL 11011-4
550349 HCV RNA PCR Qn Rfx NS5A 550074 HCV log10 log10 IU/mL 38180-6
550349 HCV RNA PCR Qn Rfx NS5A 550308 Test Information: N/A
550349 HCV RNA PCR Qn Rfx NS5A 550332 HCV NS5A Drug Resistance Assay N/A
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550075 HCV RNA (International Units) 550078 HCV RNA (International Units) IU/mL 11011-4
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550075 HCV RNA (International Units) 550081 HCV log10 log10 IU/mL 38180-6
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550316 HCV NS5A Resistance Assay PDF 72862-6
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550317 HCV NS5A Drug Resistance Assay 73655-3
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550318 HCV NS5A Resist Assay Interp 49549-9
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550316 HCV NS5A Resistance Assay PDF 72862-6
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550317 HCV NS5A Drug Resistance Assay 73655-3
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550318 HCV NS5A Resist Assay Interp 49549-9

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