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For hours, walk-ins and appointments.Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay
CPT:
87522. If reflex testing is performed, concomitant CPT codes/charges will apply.
Synonyms
- HCV Real-time PCR With Reflex to HCV NS5A Drug Resistance Assay
- HCV RNA Quantitation With Reflex to HCV NS5A Drug Resistance Assay
Test Includes
Reflex to HCV NS5A drug resistance assay if viral load is ≥500 IU/mL.
Special Instructions
Please indicate the patient's HCV genotype (1a or 1b) on the test request form.
Expected Turnaround Time
11 - 17 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Specimen Requirements
Specimen
Plasma (preferred) or serum, frozen
Volume
4 mL
Minimum Volume
2 mL
Container
Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.
Collection
Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Causes for Rejection
Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood
Test Details
Use
Quantification of HCV with reflex to HCV NS5A drug resistance assay (at additional charge) if ≥500 IU/mL.
Limitations
The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.
Methodology
cobas® HCV (Roche Molecular Systems). See Hepatitis C Virus (HCV) NS5A Drug Resistance Assay [550325] for more information.
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cobas® HCV (Roche Molecular Systems). See Hepatitis C Virus (HCV) NS5A Drug Resistance Assay [550325] for more information. |
References
Yao JD, Young S, Heilek GM, et al. Diagnosis and monitoring of HCV infection using the cobas® HCV test for use on the cobas® 6800/8800 systems. J Clin Virol. 2018 May;102:63-69.29518694
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Yao JD, Young S, Heilek GM, et al. Diagnosis and monitoring of HCV infection using the cobas® HCV test for use on the cobas® 6800/8800 systems. J Clin Virol. 2018 May;102:63-69.29518694 |
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550349 | HCV RNA PCR Qn Rfx NS5A | 11011-4 | 550073 | Hepatitis C Quantitation | IU/mL | 11011-4 |
| 550349 | HCV RNA PCR Qn Rfx NS5A | 11011-4 | 550074 | HCV log10 | log10 IU/mL | 38180-6 |
| 550349 | HCV RNA PCR Qn Rfx NS5A | 11011-4 | 550308 | Test Information: | N/A | |
| 550349 | HCV RNA PCR Qn Rfx NS5A | 11011-4 | 550332 | HCV NS5A Drug Resistance Assay | N/A |
| Reflex Table for Hepatitis C Quantitation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550075 | HCV RNA (International Units) | 550078 | HCV RNA (International Units) | IU/mL | 11011-4 |
| Reflex Table for Hepatitis C Quantitation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550075 | HCV RNA (International Units) | 550081 | HCV log10 | log10 IU/mL | 38180-6 |
| Reflex Table for HCV NS5A Drug Resistance Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550326 | HCV NS5A Drug Resistance Assay | 550316 | HCV NS5A Resistance Assay PDF | 72862-6 | |
| Reflex Table for HCV NS5A Drug Resistance Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550326 | HCV NS5A Drug Resistance Assay | 550317 | HCV NS5A Drug Resistance Assay | 73655-3 | |
| Reflex Table for HCV NS5A Drug Resistance Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 550326 | HCV NS5A Drug Resistance Assay | 550318 | HCV NS5A Resist Assay Interp | 49549-9 | |
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