Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) GenoSure® NS3/4A

CPT: 87522. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Test Details

Synonyms

  • HCV Real-time PCR With Reflex to HCV GenoSure® NS3/4A
  • HCV RNA Quantitation With Reflex to HCV GenoSure® NS3/4A

Test Includes

Reflex to HCV GenoSure® NS3/4A if viral load is ≥2000 IU/mL

Use

Quantification of HCV with reflex to HCV GenoSure NS3/4A drug resistance (at additional charge) if ≥2000 IU/mL. Detects Q80K polymorphism.

Limitations

The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.

Methodology

Cobas® AmpliPrep/Cobas® Taqman® HCV Test v 2.0. See Hepatitis C Virus (HCV) GenoSure® NS3/4A [550540] for more information.

Specimen Requirements

Specimen

Plasma, frozen

Volume

6 mL

Minimum Volume

3.3 mL

Container

PPT™ or lavender-top (EDTA) tube

Collection

Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to a screw-cap polypropylene transport tube (not "pop-top" or "snap-cap"), and freeze. Specimens collected in PPT™ need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Causes for Rejection

Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood

Clinical Information

Special Instructions

Please indicate the patient's HCV genotype (1a or 1b) on the test request form.

References

Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521

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