Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) GenoSure® NS3/4A

CPT: 87522. If reflex testing is performed, concomitant CPT codes/charges will apply.
Print Share

Synonyms

  • HCV Real-time PCR With Reflex to HCV GenoSure® NS3/4A
  • HCV RNA Quantitation With Reflex to HCV GenoSure® NS3/4A

Test Includes

Reflex to HCV GenoSure® NS3/4A if viral load is ≥2000 IU/mL


Special Instructions

Please indicate the patient's HCV genotype (1a or 1b) on the test request form.


Expected Turnaround Time

11 - 17 days


Related Documents

For more information, please view the literature below.

HCV Specimen Collection Guide


    Specimen Requirements


    Specimen

    Plasma (preferred) or serum, frozen


    Volume

    4 mL


    Minimum Volume

    2 mL


    Container

    Lavender-top (EDTA) tube, Plasma Preparation Tube (PPT™), gel-barrier tube


    Collection

    Collect specimen in two PPT™ tubes, gel-barrier tubes or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes and freeze. Specimens collected in PPT™ need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


    Storage Instructions

    Freeze.


    Causes for Rejection

    Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood


    Test Details


    Use

    Quantification of HCV with reflex to HCV GenoSure NS3/4A drug resistance (at additional charge) if ≥2000 IU/mL. Detects Q80K polymorphism.


    Limitations

    The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.


    Methodology

    Cobas® AmpliPrep/Cobas® Taqman® HCV Test v 2.0. See Hepatitis C Virus (HCV) GenoSure® NS3/4A [550540] for more information.


    References

    Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521

    LOINC® Map

    Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
    550072 HCV RNA PCR Qn Rfx NS3/4A 75888-8 550073 Hepatitis C Quantitation IU/mL 11011-4
    550072 HCV RNA PCR Qn Rfx NS3/4A 75888-8 550074 HCV log10 log10 IU/mL 38180-6
    550072 HCV RNA PCR Qn Rfx NS3/4A 75888-8 550308 Test Information: N/A
    550072 HCV RNA PCR Qn Rfx NS3/4A 75888-8 550082 HCV GenoSure(R) NS3/4A N/A
    Reflex Table for Hepatitis C Quantitation
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 550075 HCV RNA (International Units) 550078 HCV RNA (International Units) IU/mL 11011-4
    Reflex Table for Hepatitis C Quantitation
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 550075 HCV RNA (International Units) 550081 HCV log10 log10 IU/mL 38180-6
    Reflex Table for HCV GenoSure(R) NS3/4A
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 550539 Rfx to GenoSure(R) HCV NS3/4A 550544 HCV GenoSure(R) NS3/4A PDF 32286-7
    Reflex Table for HCV GenoSure(R) NS3/4A
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 550539 Rfx to GenoSure(R) HCV NS3/4A 550545 HCV GenoSure(R) NS3/4A 32286-7
    Reflex Table for HCV GenoSure(R) NS3/4A
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 550539 Rfx to GenoSure(R) HCV NS3/4A 550546 HCV GenoSure(R) NS3/4A Interp 77202-0

    For Providers

    Please login to order a test

    Order a Test

    © 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

    CPT Statement/Profile Statement

    The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf