Hepatitis C Virus (HCV) NS5B Drug Resistance Assay

CPT: 87900; 87902
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Test Details


  • HCV Drug Resistance
  • NS5B Resistance Analysis


Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5B inhibitors. Assay should be used for patients with documented HCV genotype 1.


This procedure may not be successful when the HCV viral load is <1000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.


Polymerase chain reaction (PCR) amplification and DNA next generation sequencing

Specimen Requirements


Plasma (preferred) or serum, frozen


2 mL

Minimum Volume

1 mL


Ship in plasma preparation tube (PPT™) or screw-capped polyproplylene frozen transport tubes.


Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions


Causes for Rejection

PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in "pop-top" or "snap-cap" tube

Clinical Information

Special Instructions

Please indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit a separate frozen specimen for each test ordered.


Harvoni [package insert]. Foster City, Calif; Gilead Sciences Inc; 2014.
Poveda E, Wyles DL, Mena A, Pedreira JD, Castro-Iglesias A, Cachay E. Update on hepatitis C virus resistance to direct-acting antiviral agents. Antiviral Res. 2014 Aug; 108:181-191. Erratum: Antiviral Res. 2014 Nov; 111:154. 24911972
Viekira Pak [package insert]. Highlights of Prescribing Information. Viekira PAK (ombitasvir, paritaprevir, and ritonavir tablets; dasabuvir tablets). North Chicago, Ill: AbbVie Inc; 2015.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550505 HCV NS5B Drug Resistance Assay 550506 HCV NS5B Resistance Assay PDF 72862-6
550505 HCV NS5B Drug Resistance Assay 550507 HCV NS5B Drug Resistance Assay 73655-3
550505 HCV NS5B Drug Resistance Assay 550516 HCV NS5B Resist Assay Interp 49549-9

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