Hepatitis C Virus (HCV) Genotyping With 1a Subtype Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay

CPT: 87902. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Expected Turnaround Time

12 - 18 days



Specimen Requirements


Specimen

Plasma (preferred) or serum, frozen


Volume

3 mL


Minimum Volume

2 mL


Container

Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.


Collection

Collect specimen in two PPT™ tubes, gel-barrier tubes, yellow-top (ACD) tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Freeze.


Causes for Rejection

Hemolysis; green-top (heparin) tube; specimen not frozen on date of collection; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; specimen shipped at room temperature


Test Details


Use

Genotyping of the six major HCV types and their most common subtypes. Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5A inhibitors. Detects resistance-associated variants at positions 28, 30, 31, and 93 in genotype 1a HCV.


Limitations

The HCV genotype determination may not be successful when the HCV viral load is <1000 IU/mL serum/plasma. The HCV NS5A drug resistance assay will only be performed when the HCV genotype is 1a.


Methodology

Polymerase chain reaction (PCR) amplification and solid-phase electrochemical detection


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550615 HCV Genotype Reflex NS5A 32286-7 550617 Hepatitis C Genotype 32286-7
550615 HCV Genotype Reflex NS5A 32286-7 550618 Please note: 77202-0
Reflex Table for Hepatitis C Genotype
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550316 HCV NS5A Resistance Assay PDF 72862-6
Reflex Table for Hepatitis C Genotype
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550317 HCV NS5A Drug Resistance Assay 73655-3
Reflex Table for Hepatitis C Genotype
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550318 HCV NS5A Resist Assay Interp 49549-9
Reflex Table for Hepatitis C Genotype
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550621 Reflex Test Information 550621 Reflex Test Information N/A

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