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Hepatitis C Virus (HCV) Genotyping With 1a Subtype Reflex to Hepatitis C Virus (HCV) GenoSure® NS3/4A
Genotyping of the six major HCV types and their most common subtypes. Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS3/4A protease inhibitors. Detects Q80K polymorphism.
This procedure may not be successful when the HCV viral load is <1000 IU/mL in serum or plasma.
Polymerase chain reaction (PCR) amplification and solid-phase electrochemical detection
Serum or plasma, frozen
Collect specimen in two PPT™ tubes, gel-barrier tubes, yellow-top (ACD) tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™s need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Maintain frozen at -20°C.
Causes for Rejection
Hemolysis; green-top (heparin) tube; specimen not frozen on date of collection; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; specimen shipped at room temperature
A separate frozen specimen is required for each frozen test ordered. The HCV GenoSure® NS3/4A assay is only validated for samples with HCV viral load >2000 IU/mL.