Hepatitis C Virus (HCV) GenoSure® NS3/4A

CPT: 87900; 87902
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Test Details


  • HCV Drug Resistance
  • NS3/4 Resistance Analysis
  • Q80K Polymorphism


Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS3/4A protease inhibitors. Detects Q80K polymorphism. Assay should be used for patients with documented HCV genotype 1.


This procedure may not be successful when the viral load is <2000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.


Polymerase chain reaction (PCR) amplification and DNA next generation sequencing

Specimen Requirements


Plasma (preferred) or serum, frozen


3 mL


Ship in plasma preparation tube (PPT™) or screw-capped polyproplylene frozen transport tubes.


Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions


Causes for Rejection

PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in "pop-top" or "snap-cap" tube

Clinical Information

Special Instructions

Please indicate the patient's HCV genotype (1a or 1b) on the test request form.


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Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550540 HCV GenoSure(R) NS3/4A 550544 HCV GenoSure(R) NS3/4A PDF 32286-7
550540 HCV GenoSure(R) NS3/4A 550545 HCV GenoSure(R) NS3/4A 32286-7
550540 HCV GenoSure(R) NS3/4A 550546 HCV GenoSure(R) NS3/4A Interp 77202-0

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