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Hepatitis B Virus (HBV) DNA, Quantitative Real-time PCR With Reflex to HBV Genotype Plus Drug Resistance
This test quantitates HBV DNA in serum or plasma with reflex to HBV genotype and drug resistance testing if ≥500 IU/mL is detected.
The quantitative test has a range of 10 IU/mL to 1,000,000,000 IU/mL. The genotype assay is only triggered when the quantitative test result is ≥500 IU/mL.
Quantitative test: cobas® HBV for use with cobas® 6800/8800 system
Genotype test (reflex): Polymerase chain reaction (PCR) amplification and DNA sequencing
Plasma or serum, frozen
Centrifuge sample within six hours of collection. Transfer the serum/plasma to a screw-top polypropylene tube and freeze.
Causes for Rejection
Green-top (heparin) or yellow-top (ACD) tubes used for collection; serum/plasma not frozen; PPT not centrifuged