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For hours, walk-ins and appointments.Hepatitis B Virus (HBV) DNA, Quantitative Real-time PCR With Reflex to HBV Genotype
CPT:
87517. If reflex testing is performed, concomitant CPT codes/charges will apply.
Synonyms
- HBV Quantitation
- HBV Viral Load
Expected Turnaround Time
7 - 9 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Plasma or serum, frozen
Volume
3 mL
Minimum Volume
1.5 mL
Container
Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or PPT. Do not use green-top (heparin) or yellow-top (ACD) tube
Collection
Centrifuge sample within six hours of collection. Transfer the serum/plasma to a screw-top polypropylene tube and freeze.
Storage Instructions
Freeze.
Causes for Rejection
Green-top (heparin) or yellow-top (ACD) tubes used for collection; serum/plasma not frozen; PPT not centrifuged
Test Details
Use
This test quantitates HBV DNA in serum or plasma with reflex to HBV genotype if ≥500 IU/mL is detected.
Limitations
The quantitative test has a range of 10 IU/mL to 1,000,000,000 IU/mL. The genotype assay is only triggered when the quantitative test result is ≥500 IU/mL.
Methodology
Quantitative test: cobas® HBV for use on the cobas® 6800/8800 system
Genotype test (reflex): Polymerase chain reaction (PCR) amplification and DNA sequencing
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 551722 | HBV Quant PCR Rfx to Genotype | 42595-9 | 551651 | HBV IU/mL | IU/mL | 42595-9 |
| 551722 | HBV Quant PCR Rfx to Genotype | 42595-9 | 551585 | log10 HBV as IU/mL | log10 IU/mL | 48398-2 |
| 551722 | HBV Quant PCR Rfx to Genotype | 42595-9 | 551724 | Test Information: | 19147-8 | |
| 551722 | HBV Quant PCR Rfx to Genotype | 42595-9 | 551726 | HBV Genotype | N/A |
| Reflex Table for HBV IU/mL | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551587 | HBV Real-Time PCR, Quant | 551649 | HBV IU/mL | IU/mL | 42595-9 |
| Reflex Table for HBV IU/mL | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551587 | HBV Real-Time PCR, Quant | 551586 | log10 HBV IU/mL | log10 IU/mL | 48398-2 |
| Reflex Table for HBV Genotype | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551727 | HBV Genotype | 551578 | HBV Genotype | 32366-7 | |
| Reflex Table for HBV Genotype | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551727 | HBV Genotype | 551714 | HBV PreCore Mutation | 54210-0 | |
| Reflex Table for HBV Genotype | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551727 | HBV Genotype | 551763 | HBV Genotype/PreCore | N/A | |
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CPT Statement/Profile Statement
The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf