Hepatitis B Virus (HBV) DNA, Quantitative Real-time PCR With Reflex to HBV Genotype

CPT: 87517. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Test Details

Synonyms

  • HBV Quantitation
  • HBV Viral Load

Use

This test quantitates HBV DNA in serum or plasma with reflex to HBV genotype if ≥500 IU/mL is detected.

Limitations

The quantitative test has a range of 10 IU/mL to 1,000,000,000 IU/mL. The genotype assay is only triggered when the quantitative test result is ≥500 IU/mL.

Methodology

Quantitative test: cobas® HBV for use on the cobas® 6800/8800 system

Genotype test (reflex): Polymerase chain reaction (PCR) amplification and DNA sequencing

Specimen Requirements

Specimen

Plasma or serum, frozen

Volume

3 mL

Minimum Volume

1.5 mL

Container

Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or PPT. Do not use green-top (heparin) or yellow-top (ACD) tube

Collection

Centrifuge sample within six hours of collection. Transfer the serum/plasma to a screw-top polypropylene tube and freeze.

Storage Instructions

Freeze.

Causes for Rejection

Green-top (heparin) or yellow-top (ACD) tubes used for collection; serum/plasma not frozen; PPT not centrifuged

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf