Hemoglobinopathy Fractionation Profile Without Solubility

CPT: 83021
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Test Details


  • Hemoglobin Electrophoresis

Test Includes

Identification and relative percentages of hemoglobins (A, A2, F, S, C) including variants, if present; interpretation


Diagnose thalassemias and hemoglobin variants


High-pressure liquid chromatography (HPLC)

Reference Interval

• Hb A:

– <7 months: Not established

– 7 months to 1 year: 94.6% to 98.5%

– ≥2 years: 96.4% to 98.8%

• Hb F:

− <7 months: Not established

− 7 months to 1 year: 0.1% to 6.8%

− ≥2 years : 0.0% to 2.0%

• Hb S, D, G: 0%

• Hb C, E, O: 0%

• Hb A2:

– <7 months: Not established

– 7 months to 1 year: 1.9% to 2.8%

– ≥2 years: 1.8% to 3.2%

Specimen Requirements


Whole blood


1 mL

Minimum Volume

0.5 mL


Lavender-top (EDTA) tube


To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.

Storage Instructions

Refrigerate at 2°C to 8°C.

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Clinical Information

Special Instructions

State the patient's age, ethnic background, MCV, transfusion history, and any other known hematological data on the test request form.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
122101 Hgb Frac. w/o Solubility 121706 Hgb F % 4576-5
122101 Hgb Frac. w/o Solubility 121702 Hgb A % 4546-8
122101 Hgb Frac. w/o Solubility 121703 Hgb S % 4625-0
122101 Hgb Frac. w/o Solubility 121704 Hgb C % 4563-3
122101 Hgb Frac. w/o Solubility 121705 Hgb A2 % 34660-1
122101 Hgb Frac. w/o Solubility 121707 Hgb Variant % 42248-5
122101 Hgb Frac. w/o Solubility 121708 Interpretation 12710-0
Reflex Table for Interpretation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121088 LCA Hemoglobin Lab INTERP 121089 LCA Hemoglobin Lab INTERP 12710-0

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