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FLT3 Internal Tandem Duplication (ITD) and D835 Variant Detection by PCR
- ITD and D835 Mutations
- Mutation Analysis (FLT3)
Polymerase chain reaction (PCR)/capillary electrophoresis
Whole blood or bone marrow or pellets cell suspended in cell culture medium or buffered solution or isolated DNA from peripheral blood, bone marrow, or cell pellets (without fixative)
5 mL whole blood or 3 mL bone marrow
1 mL whole blood, 1 mL bone marrow, or 0.30 mL isolated DNA
Maintain specimen at room temperature.
Causes for Rejection
Cell pellets suspended in fixatives (examples of fixative such as acetone, acetic acid, etc); DNA extracted from FFPE (formalin-fixed paraffin-embedded) tissue and bone core; any separating blood collection tubes, red-top tubes, serum or plasma and tissue; specimen more than 14 days old (unless classified as irretrievable, in which case permission from the LabPMM medical director is required to proceed with testing)