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Dihydrotestosterone (DHT), Free, LC/MS/Dialysis
- Free 5-α-dihydrotestoterone
Dihydrotestosterone measurement is used to diagnose 5α-reductase deficiency and for the evaluation of androgen utilization. Dihydrotestosterone is produced by the reduction of testosterone by 5α-reductase in the target organs. Circulating dihydrotestosterone is tightly bound to sex hormone-binding globulin. The free fraction represents the dihydrotestosterone available to act on tissues.
This test was developed, and the performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Equilibrium dialysis, high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS-MS)
Serum (preferred) or plasma, frozen
1.2 mL (Note: This volume does not allow for repeat testing.)
Separate serum or plasma from cells within four hours of collection. Send serum or plasma in a plastic transport tube. Transfer specimen to a plastic transport tube before freezing. To avoid delays in turnaround time when requesting multiple test on frozen samples, please submit separate frozen specimens for each test requested.
Freeze. Stable at room temperature, refrigerated, or frozen for 14 days. Freeze/thaw cycles: stable x6
Causes for Rejection
EDTA plasma; grossly hemolyzed, icteric, or lipemic sample