11-Deoxycortisol, LC/MS-MS (Endocrine Sciences)

CPT: 82634
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Test Details

Synonyms

  • 11-Deoxycortisol
  • Compound S

Use

Evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve. 11-Deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol.

Methodology

Liquid chromatography/tandem mass spectrometry (LC/MS-MS) after chromatography and solvent extraction

Reference Interval

See table.

Premature Infants

26 to 28 wk, day 4

110−1376 ng/dL

31 to 35 wk, day 4

48−579 ng/dL

Full-term Infants

3 d

13−147 ng/dL

31 d to 11 m

<10−156 ng/dL

Prepubertal Children

8 AM

20−155 ng/dL

Pubertal Children and Adults

8 AM

12−158 ng/dL

Additional Information

A primary use of 11-desoxycortisol testing is to detect congenital adrenal hyperplasia due to steroid 11-β-hydroxylase deficiency. This is a genetic disease caused by a deficiency of the CYP11B1 gene. 11-β-hydroxylase deficiency is transmitted as an autosomal recessive trait. It is associated with low-renin hypertension, hypokalemia, excess androgens (especially androstenedione), and genital ambiguity in affected females. The differential biochemical diagnosis of congenital adrenal hyperplasia caused by 11-β-hydroxylase deficiency includes finding increased serum concentrations of deoxycorticosterone, 11-desoxycortisol and androstenedione, and suppressed plasma renin concentrations. The disorder may be treated by administration of glucocorticoids. An additional use is to evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve. 11-deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol.

Specimen Requirements

Specimen

Serum (preferred) or plasma

Volume

1 mL

Minimum Volume

0.2 mL

Container

Gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube

Collection

Serum/plasma must be separated from cells within 45 minutes of venipuncture. Send serum/plasma in a plastic transport tube.

Storage Instructions

Freeze.

Stability Requirements

Temperature

Period

Room temperature

1 day

Refrigerated

1 day

Frozen

2 years

Freeze/thaw cycles

Stable x3

Clinical Information

Special Instructions

For information on metyrapone testing, consult the Metyrapone Stimulation section of the Endocrine Appendix.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
500171 11-Desoxycortisol 504584 11-Desoxycortisol ng/dL 1657-6
500171 11-Desoxycortisol 500907 11-Desoxycortisol N/A

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