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For hours, walk-ins and appointments.Chromosome Analysis, AFP, AChE, Amniotic Fluid With Reflex to Fetal Hemoglobin (Hb F)
CPT:
82013; 82106. If additional testing is performed, concomitant CPT codes/charges will apply.
Test Includes
Analysis of fetal karyotype, AFP, and AChE to rule out chromosomal abnormalities and open neural tube defects (ONTD). The Hb F reflex is used to ascertain false elevations of amniotic fluid α-fetoprotein due to fetal blood contamination.
Special Instructions
State gestational age (in weeks) on the day the sample was drawn on the test request form. The patient's gestational age must be between 12 weeks and 24 weeks for interpretation of AF-AFP. AChE false-positive and false-negative results are more frequent at gestational ages of less than 13 weeks or greater than 24.9 weeks.
If prior NIPT studies have been performed, include copy of the report.
Expected Turnaround Time
9 - 12 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Specimen Requirements
Specimen
Amniotic fluid
Volume
20 to 30 mL
Minimum Volume
10 mL
Container
Sterile plastic conical tube; do not use urine containers or tubes with rubber stoppers. Rubber is toxic to amniocytes.
Collection
Discard first 2 mL of fluid aspirated to avoid maternal cell contamination. Specimen is collected in a 20 mL sterile syringe and transferred aseptically to sterile tubes. The test request form is completed and accompanies specimen and miscellaneous slip to the laboratory.
Storage Instructions
Maintain specimen at room temperature. Do not freeze or refrigerate.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 1 month |
Causes for Rejection
Specimen found not to be amniotic fluid; gross contamination with red cells; frozen specimen; nonsterile container. Do not use urine containers or tubes with rubber stoppers; rubber is toxic to amniocytes.
Test Details
Use
Determine fetal karyotype; prenatal diagnosis of Down syndrome or other chromosomal abnormalities; analysis of midtrimester amniotic fluid for detection of open neural tube and ventral wall defects
Methodology
In situ cell culturing of amniocytes for the microscopic investigation of numerical and/or structural chromosome abnormalities; chemiluminescent immunoassay for AFP; isoelectric focusing (IEF); acrylamide gel electrophoresis
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052138 | Cells Counted | 64095-3 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052139 | Colonies | 64096-1 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052140 | Cells Analyzed | 64092-0 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052141 | Cells Karyotyped | 64091-2 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052143 | GTG Band Resolution Achieved | 64093-8 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052144 | Cytogenetic Diagnosis | 62356-1 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052033 | Cytogenetic Interpretation | 62357-9 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052146 | Specimen Type | 48002-0 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 052034 | Director Review: | 48672-0 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 512123 | 51967-8 | |||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 010806 | AFP, Amniotic Fluid | N/A | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 101352 | AFP Value | ug/mL | 1832-5 | |
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 101360 | AFP MoM | 29253-2 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 101379 | Gestational Age(Wks) | 49051-6 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 101378 | Interpretation | 41273-4 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 101376 | Director | 72486-4 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 999500 | Amniotic Fluid, AFP | N/A | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 510172 | Acetylcholinesterase | 30106-9 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 510173 | Interpretation | 76479-5 | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 510177 | ACHE | N/A | ||
| 511580 | Chromosome, AFP/AChE/HbF, Amn | 510170 | Fetal Hemoglobin Testing | N/A |
| Reflex Table for Interpretation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 510206 | AChE+Hb F | 510172 | Acetylcholinesterase | 30106-9 | |
| Reflex Table for Interpretation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 510206 | AChE+Hb F | 510180 | Fetal Hemoglobin | 28067-7 | |
| Reflex Table for Interpretation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 510206 | AChE+Hb F | 510173 | Interpretation | 76479-5 | |
| Reflex Table for Fetal Hemoglobin Testing | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 510181 | Fetal Hemoglobin | 510180 | Fetal Hemoglobin | 28067-7 | |
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