Chimerism Analysis With CD33/CD66b Cells (Transplant Monitoring)

CPT: 81268(x2)
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Test Details

Synonyms

  • Cell Sorting CD33/CD66b
  • Chimerism Analysis With Sorted Myeloid Cells
  • Engraftment Monitoring Sorted Myeloid Cells

Use

Monitor the level of donor CD33/CD66b cells in the recipient (patient-host) of a hematopoietic stem cell transplant. Polymorphic loci determined in the preëngraftment testing are used as a reference for the evaluation.

Limitations

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Methodology

Antibody-mediated positive selection of CD33/CD66 cells and separation with magnetic nanoparticles. DNA analysis by polymerase chain reaction (PCR) of short tandem repeats (STR)

Specimen Requirements

Specimen

Whole blood

Volume

6 mL

Minimum Volume

2 mL

Container

Yellow-top (ACD-A or ACD-B) tube

Patient Preparation

None

Storage Instructions

Maintain specimen at room temperature. Do not refrigerate or freeze.

Causes for Rejection

Improper collection tube (ie, EDTA or heparin); hemolysis; clotted specimen

Clinical Information

Special Instructions

To schedule this test, you must telephone 800-533-1037, HLA customer service. Ship specimen to arrive in the laboratory between Monday and Friday.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
176079 Chimerism Anal CD33/CD66b Cell 176138 Donor 1 N/A
176079 Chimerism Anal CD33/CD66b Cell 176373 Donor 2 N/A
176079 Chimerism Anal CD33/CD66b Cell 176374 Donor 3 N/A
176079 Chimerism Anal CD33/CD66b Cell 176375 Donor 4 N/A

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2017, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf