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Evaluate amniotic fluid for the presence of RBCs (semiquantitative), WBCs, differential, and for physical appearance
Leukocyte (WBC) count is often of limited value; however, amniotic fluid infection or chorioamnionitis is usually associated with polymorphonuclear leukocytosis (>100/μL). Controversy exists in the literature concerning the significance of the presence of leukocytes in amniotic fluid.1,4,5 Some studies reflect the absence of leukocytes and bacteria was associated with a negative amniotic fluid culture in 77.9% to 95% of the patients.1,4,6
Microscopic and macroscopic examination
Amniotic, amniocentesis fluid
1 to 2 mL
0.5 mL (Note: This volume does not allow for repeat testing.)
Sterile container or lavender-top (EDTA) tube or green-top (heparin) tube
Prior to amniocentesis, an ultrasound examination should be performed.1-3 Ultrasound examination is used to:
• Confirm fetal gestational age
• Detect gross fetal anomalies
• Verify fetal cardiac activity
• Determine placental location
• Determine volume of amniotic fluid
• Determine the number of gestational sacs
• Determine the correct abdominal puncture site
The first 1-2 mL should be discarded due to contamination with blood or maternal cells. When blood-tinged fluid is obtained initially, another syringe should be used to obtain clear fluid, if possible.
Refrigerate at 2°C to 8°C; stable 24 hours. Transport to the laboratory as soon as possible.
Causes for Rejection
Room temperature storage; frozen specimen; specimen more than 24 hours old; improperly labeled specimen container
Specimens referred to our laboratories for fluid cell counts must be handled properly to ensure accuracy. Please call for handling instructions.
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|005260||Cell Ct, Amniotic Fluid||005261||Color, Amniotic||38386-9|
|005260||Cell Ct, Amniotic Fluid||005262||Clarity, Amniotic||9335-1|
|005260||Cell Ct, Amniotic Fluid||005269||Nucleated Cells, Amniotic||/uL||55793-4|
|005260||Cell Ct, Amniotic Fluid||005265||RBC, Amniotic||/uL||55779-3|
|005260||Cell Ct, Amniotic Fluid||005266||Polys, Amniotic||%||26518-1|
|005260||Cell Ct, Amniotic Fluid||005267||Mononuclear Cells, Amniotic||%||26493-7|
|005260||Cell Ct, Amniotic Fluid||005268||Comments:||20999-9|