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- BV (Sialidase) Activity
Enzyme activity test for use in the detection of vaginal fluid specimens for sialidase activity, an enzyme produced by bacteria associated with bacterial vaginitis, including Bacteroides spp, Prevotella spp, and Mobiluncus spp and some G vaginalis. In a recent study, Bradshaw et al demonstrated the test to be 88% sensitive and 95% specific.
Collecting specimens from the cervix should be avoided because (a) it might increase risk to OB patients, and (b) cervical sialidase activity is usually higher than vaginal sialidase activity. Do not collect specimens from patients who have (a) used a vaginal cream or ointment product, (b) douched, or (c) used spermicides, vaginal lubricants, or feminine sprays within 72 hours of testing. If insufficient sample is collected or collected from a patient undergoing antimicrobial therapy, the test may give a false-negative result.
Chromogenic enzyme activity test
Copan double swab in Amies liquid medium
Using transport swabs provided, obtain a sample by contacting the lower one-third of the vaginal wall, rotating 10 to 20 seconds to absorb fluid. Immediately place the swabs into the transport tube.
Maintain specimen at room temperature for up to 48 hours.
Causes for Rejection
Specimen collected or transported other than previously described
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|180034||BV, Sialidase Activity||41477-1||180035||BV, Sialidase Activity||41477-1|