Bacterial Stool Pathogens (Salmonella sp, Shigella sp, Campylobacter sp, E coli [STEC]), NAA

CPT: 87505
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Test Details

Test Includes

Campylobacter sp; E coli (STEC); Salmonella sp; Shigella sp

Use

Determine the presence of bacterial pathogens in the stool of patients with gastrointestinal symptoms.

Limitations

This test detects C jejuni and C coli but does not differentiate between these two species of Campylobacter. Antibiotic susceptibility testing is not performed since bacterial isolates are not generated by this methodology.

Methodology

Nucleic acid amplification (NAA)

Specimen Requirements

Specimen

Stool

Volume

1 C&S vial (Para-Pak orange)

Minimum Volume

0.5 mL

Collection

Remove cap and place approximately 1 gram of the raw stool into the transport medium or a sufficient amount to bring the liquid level up to the "fill to here" line. Replace cap and tighten. Agitate the vial to permit adequate mixing of the specimen with the transport medium. When mixing is completed, the solution should appear homogeneous.

Storage Instructions

Maintain specimen at room temperature for five days or refrigerated for 14 days. Do not freeze.

Causes for Rejection

Raw stool; inappropriate specimen transport device; specimen beyond stated stability; frozen specimen; specimens with incorrect or missing patient information; specimen in expired transports

Clinical Information

Special Instructions

Standard stool collection and handling procedures are appropriate to obtain liquid or soft raw stool. Specimens must be collected into a sterile screw-cap container and then transferred into the C&S transport vial with Cary Blair medium (Para-Pak® orange) within two hours of collection.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
183170 Bacterial Stool Pathogens, NAA 183171 Salmonella sp 49612-5
183170 Bacterial Stool Pathogens, NAA 183172 Shigella sp 46455-2
183170 Bacterial Stool Pathogens, NAA 183173 Campylobacter sp 71429-5
183170 Bacterial Stool Pathogens, NAA 183174 E. coli STEC 81285-9

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