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For hours, walk-ins and appointments.α-Fetoprotein (AFP), AChE, Amniotic Fluid With Reflex to Fetal Hemoglobin (Hb F)
CPT:
82013; 82106
Synonyms
- AFP
- Alpha-Fetoprotein, AChE, Hb F, Amniotic Fluid
Test Includes
Measurement of amniotic fluid AFP and detection of AChE. The reflex to fetal hemoglobin (Hb F) will be used, if necessary, to ascertain false elevations of amniotic fluid AFP due to contamination with fetal blood.
Special Instructions
Patient's gestational age must be between 12 weeks and 24 weeks for interpretation of α-fetoprotein. State gestational age by ultrasound and/or last menstrual period (LMP) on the request form. Note that AChE false-positive and false-negative results are more frequent at gestational ages of less than 13 weeks or greater than 24.9 weeks. Optimal gestational age for detection of AChE is 14 to 18 weeks.
Expected Turnaround Time
4 - 10 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Specimen Requirements
Specimen
Amniotic fluid
Volume
3 mL
Container
Sterile plastic conical tube; do not use urine containers or tubes with rubber stoppers. Rubber is toxic to amniocytes.
Collection
Avoid contamination of amniotic fluid with maternal or fetal blood. As little as one drop of fetal blood can cause false-positive results during assay of amniotic fluid. Amniotic fluid should be collected by the attending physician.
Storage Instructions
Ship and maintain specimen at room temperature.
Patient Preparation
The patient should have undergone ultrasound studies to verify fetal viability, detect multiple gestation, confirm gestational age, localize placenta, and detect fetal and uterine pathology.
Causes for Rejection
Specimen found not to be amniotic fluid; gross contamination of amniotic fluid with maternal or fetal blood; quantity not sufficient for analysis
Test Details
Use
Analysis of midtrimester amniotic fluid for detection of open neural tube and ventral wall defects
Limitations
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Methodology
Chemiluminescent immunoassay; acrylamide gel electrophoresis; isoelectric focusing
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 010806 | AFP, Amniotic Fluid | N/A | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 101352 | AFP Value | ug/mL | 1832-5 | |
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 101360 | AFP MoM | 29253-2 | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 101379 | Gestational Age(Wks) | 49051-6 | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 101378 | Interpretation | 41273-4 | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 101376 | Director | 72486-4 | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 999500 | Amniotic Fluid, AFP | N/A | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 510172 | Acetylcholinesterase | 30106-9 | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 510173 | Interpretation | 76479-5 | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 510177 | ACHE | N/A | ||
| 510305 | AFP/AChE/HbF, Amniotic Fluid | 510170 | Fetal Hemoglobin Testing | N/A |
| Reflex Table for Interpretation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 510206 | AChE+Hb F | 510172 | Acetylcholinesterase | 30106-9 | |
| Reflex Table for Interpretation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 510206 | AChE+Hb F | 510180 | Fetal Hemoglobin | 28067-7 | |
| Reflex Table for Interpretation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 510206 | AChE+Hb F | 510173 | Interpretation | 76479-5 | |
| Reflex Table for Fetal Hemoglobin Testing | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 510181 | Fetal Hemoglobin | 510180 | Fetal Hemoglobin | 28067-7 | |
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