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- Flow Crossmatch
- HLA Crossmatch
- T-Cell and B-Cell Crossmatch
Detection of the presence of antidonor antibodies (HLA) in a potential solid organ recipient, T- and B-cell crossmatch detects the presence of anti-Class I/Class II HLA antibodies in the recipient that would recognize donor-specific antigens. The presence of antibodies against donor HLA antigens is a contraindication for transplantation and could lead to immediate/acute organ rejection.
Although the primary antibodies that are detected by this assay are to HLA, other non-HLA-specific antibodies could cause positive T- and/or B-cell crossmatch. This testing only demonstrates antibodies that recognize donor antigens and cannot distinguish clinical relevant antibodies (eg, complement binding ability) from nonclinical antibodies. This assay only detects the presence of IgG antidonor antibodies and does not detect IgM or other immunoglobulin subclasses.
This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Presence of antibody bound to human lymphocytes detected by immunofluorescence and flow cytometry
Donor sample: Whole blood; Recipient sample: Serum
Donor sample: 12 mL; Recipient sample: 2 mL
Donor sample: 8 mL; Recipient sample: 2 mL
Donor sample: Yellow-top (ACD) tube (solution A or B). Recipient sample: Red-top tube or gel-barrier tube
Maintain whole blood and serum at room temperature. Donor specimens should be analyzed within 72 hours of collection. Recipient serum may be stored at 4°C for one week or at -20°C indefinitely.
Causes for Rejection
EDTA or heparin tube; hemolysis; clotted specimen; donor samples exceeding 72 hours
To schedule this test, you must telephone 800-533-1037, HLA customer service.
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|167160||Allo-Crossmatch by Flow||167191||AlloCrossmatch T-Cell(ALTCELL)||48409-7|
|167160||Allo-Crossmatch by Flow||167192||AlloCrossmatch B-Cell(ALBCELL)||48390-9|