Acetylcholinesterase (AChE), Amniotic Fluid With Reflex to Fetal Hemoglobin (Hb F)

CPT: 82013
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Test Details


  • AChE
  • Amniotic Fluid With Reflex to Hb F

Test Includes

Detection of AChE. The reflex to fetal hemoglobin (Hb F) will be performed, if necessary, to ascertain false elevations of amniotic fluid AFP.


Analysis of midtrimester amniotic fluid for detection of open neural tube defects and ventral wall defects


Acrylamide gel electrophoresis; isoelectric focusing (IEF)

Specimen Requirements


Amniotic fluid


3 mL


Sterile plastic conical tube; do not use urine containers or tubes with rubber stoppers. Rubber is toxic to amniocytes.

Patient Preparation

The patient should have undergone ultrasound studies to verify fetal viability, detect multiple gestation, confirm gestational age, localize placenta, and detect fetal and uterine pathology.


Avoid contamination of amniotic fluid with maternal or fetal blood. As little as one drop of fetal blood can cause false-positive results during assay of amniotic fluid. Amniotic fluid should be collected by the attending physician.

Storage Instructions

Ship and maintain specimen at room temperature.

Causes for Rejection

Specimen found not to be amniotic fluid; gross contamination of amniotic fluid with maternal or fetal blood; quantity not sufficient for analysis

Clinical Information

Special Instructions

The patient's gestational age must be at least 13 weeks for accurate AChE detection. Optimal gestational age is 14 to 18 weeks. Include the gestational age by ultrasound and/or last menstrual period (LMP) on the request form.

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