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Acetylcholinesterase (AChE), Amniotic Fluid With Reflex to Fetal Hemoglobin (Hb F)
- Amniotic Fluid With Reflex to Hb F
Detection of AChE. The reflex to fetal hemoglobin (Hb F) will be performed, if necessary, to ascertain false elevations of amniotic fluid AFP.
Analysis of midtrimester amniotic fluid for detection of open neural tube defects and ventral wall defects
Acrylamide gel electrophoresis; isoelectric focusing (IEF)
Avoid contamination of amniotic fluid with maternal or fetal blood. As little as one drop of fetal blood can cause false-positive results during assay of amniotic fluid. Amniotic fluid should be collected by the attending physician.
Ship and maintain specimen at room temperature.
Causes for Rejection
Specimen found not to be amniotic fluid; gross contamination of amniotic fluid with maternal or fetal blood; quantity not sufficient for analysis
The patient's gestational age must be at least 13 weeks for accurate AChE detection. Optimal gestational age is 14 to 18 weeks. Include the gestational age by ultrasound and/or last menstrual period (LMP) on the request form.