Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to HIV-1 GenoSure PRIme® or GenoSure Archive®

CPT: 87536
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Synonyms

  • HIV-1 Plasma Viremia
  • HiV-1 RNA Real-time PCR, Quantitative
  • Reflex to GenoSure Archive®
  • Reflex to GenoSure PRIme®
  • Viral Load

Special Instructions

If reflex test is performed, additional charges/CPT code(s) may apply.


Related Documents


Specimen Requirements


Specimen

Plasma and whole blood


Volume

5 mL plasma and 4 mL EDTA whole blood


Minimum Volume

3.5 mL plasma and 3 mL EDTA whole blood


Container

Lavender-top (EDTA) tube, screw-capped polypropylene frozen transport tube


Collection

Both frozen plasma and frozen whole blood are required for this profile. Draw three lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes.

Plasma: Centrifuge two tubes within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship specimen frozen.

Whole blood: Do not centrifuge. Freeze tube immediately after draw. Ship specimen frozen.

To avoid delays in turnaround time, when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Freeze.


Stability Requirements

Frozen: 6 weeks


Causes for Rejection

Insufficient volume; heparinized plasma; specimen not frozen


Test Details


Use

Detect and measure HIV-1 in plasma with reflex to GenoSure PRIme® if viral load is greater than or equal to 500 copies/mL or reflex to GenoSure Archive® if viral load is less than 500 copies/mL. The GenoSure PRIme® and GenoSure Archive® assays detect mutations in the HIV viral genes that encode for HIV protease, reverse transcriptase and integrase. Mutations in these genes have been associated with resistance to antiretroviral drugs.


Limitations

This procedure should only be used for patients with a documented HIV-1 infection.


Methodology

Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0 and polymerase chain reaction (PCR) amplification and DNA sequencing


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550675 RNA PCR NonGraph Rfx PRIme/Arc Pending 550445 HIV-1 RNA by PCR copies/mL 20447-9
550675 RNA PCR NonGraph Rfx PRIme/Arc Pending 550446 log10 HIV-1 RNA log10copy/mL 29541-0
550675 RNA PCR NonGraph Rfx PRIme/Arc Pending 550703 Reflex to PRIme or Archive N/A
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 552039 GenoSure PRIme(R) 552019 PDF 51969-4
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 552039 GenoSure PRIme(R) 552021 HIV GenoSure PRIme(R) 80689-3
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 552039 GenoSure PRIme(R) 552022 HIV GenoSure PRIme(R) Interp 62364-5
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551798 GenoSure Archive(R) 551774 PDF 80695-0
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551798 GenoSure Archive(R) 551777 HIV GenoSure Archive 93047-9
Reflex 2 551781 HIV GSArchive 551781 HIV GSArchive N/A
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551798 GenoSure Archive(R) 551778 HIV GenoSure Archive 80693-5
Reflex 2 551782 HIV GSArchive Interp 551782 HIV GSArchive Interp N/A
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551798 GenoSure Archive(R) 551779 HIV GenoSure Archive Interp 80694-3

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