Rheumatoid Factor, IgA by EIA (RDL)

CPT: 86431
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Test Includes

This test is a component of Rheumatoid Factor Isotypes, IgG, IgA and IgM by EIA.


Expected Turnaround Time

5 - 7 days


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Specimen Requirements


Specimen

Serum


Volume

1 mL


Minimum Volume

0.3 mL (Note: This volume does not allow for repeat testing.)


Collection

Separate serum from cells within one hour. Transfer to a plastic transport tube before freezing.


Storage Instructions

Store frozen or refrigerated.


Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

14 days

Frozen

60 days

Freeze/thaw cycles

Stable x1


Causes for Rejection

Grossly hemolyzed; bacterial contamination; lipemic, icteric, non-serum specimen types


Test Details


Use

EIA methods have the added advantage of being able to simultaneously detect RF of IgG and IgA subclasses in addition to RF IgM and are not susceptible to prozone. It has become apparent that the specificity and predictive value of the RF test is substantially increased by the detection of all three RF isotypes.


Methodology

Enzyme-linked immunosorbent assay (ELISA)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
520101 RF, IgA by EIA (RDL) 33313-8 520102 RF, IgA by EIA (RDL) U 33313-8

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