Anti-Nuclear Antibodies by Indirect Fluorescent Antibody (IFA) (RDL)

CPT: 86038
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Synonyms

  • ANA by IFA
  • ANA by Immunofluorescence Antibody
  • ANA indirect
  • IFA titer and pattern

Expected Turnaround Time

5 - 7 days


Related Documents

For more information, please view the literature below.

Antinuclear Antibodies Testing Options in Rheumatology Brochure


Specimen Requirements


Specimen

Serum


Volume

1 mL


Minimum Volume

0.3 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Collection

Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Refrigerate or freeze.


Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

14 days

Frozen

60 days

Freeze/thaw cycles

Stable x1


Causes for Rejection

Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types


Test Details


Use

ANA are commonly found in a variety of autoimmune diseases. Antibody frequency increases with age in apparently healthy people. ANA patterns on Hep-2 slides provide only general clues about particles (chromatin, nucleosomes, and spliceosomes). ANA pattern (other than centromere pattern) are not reliably correlated with the presence of specific antibodies and must be further evaluated by EIA using individual ENA antigens.


Limitations

This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.


Methodology

Indirect Immunofluorescence (IFA)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
520075 Anti-Nuclear Ab by IFA (RDL) 42254-3 520073 Anti-Nuclear Ab by IFA (RDL) 42254-3
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520074 Homogeneous Pattern 20398-4
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520076 Nucleolar Pattern 20399-2
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520233 Speckled Pattern 20401-6
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520234 Centromere Pattern 53993-2
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520235 Spindle Apparatus Pattern 53003-0
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520236 Nuclear Membrane Pattern 54006-2
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520237 Midbody Pattern 54155-7
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520238 Nuclear Dot Pattern 53998-1
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520239 PCNA Pattern 53006-3
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520240 Centriole Pattern 53993-2
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520077 ANA Titer and Pattern 520241 Note: 77202-0
Reflex Table for Anti-Nuclear Ab by IFA (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520218 Anti-Ro (SS-A) Ab (RDL) 520218 Anti-Ro (SS-A) Ab (RDL) Units 33569-5

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