Mycobacterium marinum Susceptibility (AST)−Broth Dilution

CPT: 87186
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Test Includes

Susceptibility testing for amikacin, ciprofloxacin, clarithromycin, doxycycline, linezolid, minocycline, moxifloxacin, rifabutin, rifampin, streptomycin, trimethoprim/sulfamethoxazole. MIC values will be reported with CLSI interpretive comments if available.


Expected Turnaround Time

21 - 28 days



Related Documents


Specimen Requirements


Specimen

Mycobacterium marinum isolate from a primary clinical specimen should be submitted on conventional solid or broth AFB broth medium.


Volume

Pure culture isolate on an AFB conventional solid medium or a minimum of 1 mL of AFB broth medium


Container

Conventional or broth medium, tightly sealed, in etiologic agent packaging


Storage Instructions

Maintain media at room temperature.


Causes for Rejection

Specimen received in leaking or in broken transport tube or vial; specimen received in expired transport medium; mixed culture; unlabeled culture or name discrepancy between specimen and request label


Test Details


Use

Determine the susceptibility of Mycobacterium marinum isolates to relevant antimycobacterial agents. Routine susceptibility testing of M. marinum isolates is generally not recommended.1,2 The following agents are typically found to be susceptible when testing M. marinum: Rifampin, Ethambutol, Doxycycline, Minocycline, Clarithromycin and Trimethoprim-sulfamethoxazole.3 Disease due to M. marinum is generally localized and the number of organisms is low, with 95% of tissue biopsies reported as AFB smear negative. Susceptibility testing should be considered when patients remain culture positive after several months of therapy.4


Limitations

Susceptibilities cannot be reported if the organism fails to grow in the test medium. Susceptibilities cannot be performed on mixed cultures.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.


Methodology

Sensititre® broth microdilution (MIC)


Additional Information

Few comparative studies have been performed relating clinical outcome with in vitro susceptibility testing. Ciprofloxacin is not recommended for therapy because some strains are resistant and with monotherapy, the risk of mutational resistance increases.


Footnotes

1. Diagnosis and treatment of disease caused by nontuberculous mycobacteria. Am J Resp Crit Care Med. 1997 Aug;156(2 Pt 2):S1-S25.9279284
2. Clinical Laboratory Standards Institute (CLSI). Performance Standards for Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes. 1st ed. CLSI supplement M62. Wayne, PA: CLSI; 2018.
3. Griffith DE, Aksamit T, Brown-Elliott BA, et al. An official ATS/IDSA statement: Diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med. 2007 Feb 15;175(4):367-416.17277290
4. Woods GL, Lin SYG, Brown BA, Desmond EP. Susceptibility Test Methods: Mycobacteria, Nocardia and Other Actinomycetes. Manual of Clinical Microbiology,12th ed. 2018; 78:1398-1419.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
182840 M. marinum Susceptibility 29576-6 182839 Organism ID 9825-1
182840 M. marinum Susceptibility 29576-6 998549 Amikacin 12-5
182840 M. marinum Susceptibility 29576-6 998707 Ciprofloxacin 185-9
182840 M. marinum Susceptibility 29576-6 998550 Clarithromycin 189-1
182840 M. marinum Susceptibility 29576-6 998246 Clofazimine 16623-1
182840 M. marinum Susceptibility 29576-6 998720 Doxycycline 225-3
182840 M. marinum Susceptibility 29576-6 998711 Linezolid 29254-0
182840 M. marinum Susceptibility 29576-6 998552 Minocycline 335-0
182840 M. marinum Susceptibility 29576-6 998712 Moxifloxacin 31037-5
182840 M. marinum Susceptibility 29576-6 998241 Rifabutin 16100-0
182840 M. marinum Susceptibility 29576-6 998553 Rifampin 428-3
182840 M. marinum Susceptibility 29576-6 998714 Streptomycin 460-6
182840 M. marinum Susceptibility 29576-6 998554 Trimethoprim/Sulfa 516-5
Reflex Table for Organism ID
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 000000 Antimicrobial Susceptibility 000000 Antimicrobial Susceptibility N/A

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