BK Virus (BKV), Urine, Quantitative PCR

CPT: 87799
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Synonyms

  • Polyomavirus BK Quantitation
  • Quantitation of BK Virus DNA PCR, Urine

Special Instructions

Urine samples must be transferred to the cobas® PCR Urine Sample Kit within 24 hours of collection.


Expected Turnaround Time

3 - 4 days


Related Documents


Specimen Requirements


Specimen

Urine, stabilized in cobas® PCR Media


Volume

Fill urine to fall within the 2 black lines on the cobas® PCR Media tube.


Minimum Volume

Fill urine to fall within the 2 black lines on the cobas® PCR Media tube.


Container

cobas® PCR Media


Collection

Collect a urine sample in a sterile cup. Transfer the urine using the transfer pipette included in the cobas® PCR Urine Sample Kit to the cobas® PCR Media tube. Transfer enough urine so the level falls between the two black lines on the cobas® PCR Media tube. Cap the tube tightly and invert 5 times to mix the sample.


Storage Instructions

Submit specimen in cobas® PCR Media tubes under ambient or refrigerated conditions. Do not freeze.


Stability Requirements

Temperature

Period

Room temperature

90 days (stabilized in cobas® PCR Media)

Refrigerated

90 days (stabilized in cobas® PCR Media)

Frozen

Unstable


Causes for Rejection

Urine not submitted in cobas® PCR Media; under-filled or over-filled cobas® PCR Media tubes; leaking or broken tube; inadequate storage or transport


Test Details


Use

Detection and quantitation of BK Virus (BKV) in urine stabilized in cobas® PCR Media can be used as an aid in the diagnosis and management of BK virus infections. The assay is traceable to the 1st WHO International Standard for BK Virus Nucleic Acid Amplification Techniques (NAT)-based assays.


Limitations

The linear range of the assay is 200-100,000,000 IU/mL. The analytical sensitivity is 12.2 IU/mL.


Methodology

Real-time polymerase chain reaction (PCR)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
138880 BKV Quant PCR (Urine) 41480-5 139816 BKV DNA, Quant PCR, Urine IU/mL 41480-5
138880 BKV Quant PCR (Urine) 41480-5 139817 log10 BKV DNA, Urine log10 IU/mL 90924-2
Reflex Table for BKV DNA, Quant PCR, Urine
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 139820 BKV Quant PCR (Urine) 139821 BKV DNA, Quant PCR, Urine IU/mL 41480-5
Reflex Table for BKV DNA, Quant PCR, Urine
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 139820 BKV Quant PCR (Urine) 139822 log10 BKV DNA, Urine log10 IU/mL 90924-2

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