Pap Test Terminology
In 2014, a task force updated the classification system for reporting Pap test or smear results, called the Bethesda System. The Bethesda System terminology was originally developed in 1988. The 2014 review of the system incorporated minimal changes to the earlier version but refined some positions and content.
Bethesda System Terminology:
Important Sections of the Report and the New Terminology
Specimen Adequacy: Each laboratory must report if the cervical sample obtained for the test was adequate and if the quality of the smear was satisfactory for examining under the microscope. Before looking at the results, one should always check to see if the smear has been marked "Satisfactory." If "unsatisfactory," the reasons will be given and the smear will have to be repeated in 2 or 3 months.
Interpretation/Results: This is the section where the important findings from looking at the cells are reported. There are four different sections:
- Negative for intraepithelial lesion or malignancy
- Other: Endometrial cells present in a woman age 40 or more
- Epithelial Cell Abnormalities
- Other Malignancies
Negative for intraepithelial lesion or malignancy:
This is the desired result and where a "normal" result is reported. There are two important subcategories, where abnormal findings not related to the risk of cancer are reported. These are:
- ORGANISMS: This is where evidence of trichomonas, a fungal (yeast) infection, herpes, or some other infection is reported.
- OTHER NON-NEOPLASTIC FINDINGS: This is where evidence of injury and response to injury, previously termed "Benign Cellular Changes," is reported.
Other: Endometrial cells present in a woman age 40 or more:
This section is used to alert the health practitioner that endometrial cells (cells from the lining of the uterus) are present when they normally should not be. This is a check on the status of the uterus and endometrium and not the cervix. When a woman is having monthly periods, endometrial cells are often present near the time of a period. After menopause, however, they should not be present. If you are still having periods even though you are over age 40, you and your healthcare provider can disregard this part of the report.
Epithelial Cell Abnormality:
This section is where abnormalities that are associated with the risk of developing cancer are reported. The abnormalities range from changes that are only slightly abnormal to definite cancer. There is a spectrum of change. There are two types of epithelial cells in the cervix (squamous and glandular) and changes in each are listed.
Squamous Abnormalities (cells that cover most of the external part of the cervix)
- Atypical Squamous Cells: Unknown Significance (ASC-US) or Cannot exclude HSIL or high-grade changes (ASC-H)
- Low Grade Squamous Intraepithelial Lesion (LSIL)
- High Grade Squamous Intraepithelial Lesion (HSIL); one subcategory: "with features suspicious for malignancy"
- Squamous Cell Carcinoma
The potential for malignancy increases as you move down the list to the last diagnosis of Squamous Cell Carcinoma, which is an invasive cancer.
Note: There are over 50 million Pap smears done in the U.S. each year and approximately 4,000 new cancers are reported. The odds of a positive Pap smear for cancer are quite small.
Glandular Abnormalities (cover the lining of the uterus opening and canal)
- Atypical cells, not otherwise specified
- Atypical cells, favor neoplastic
- Adenocarcinoma in situ
- Adenocarcinoma (can be endometrial (uterus), endocervical (cervix), extrauterine (origin from outside uterus and cervix), or the site of the malignancy cannot be determined based on the Pap smear))
Glandular abnormalities are much less common than squamous abnormalities. The list above is arranged so that the potential for malignancy increases as you move down the list. A diagnosis like adenocarcinoma in situ (a cancer limited to the surface that has not invaded) is one of the rarest diagnoses made on a Pap smear and frequently requires consultation among pathologists.
Malignant tumors other than primary squamous carcinoma and glandular adenocarcinoma are occasionally seen on a Pap smear and they are reported here.
It is important that if you do not understand your Pap smear results, you ask your healthcare provider for clarification. Ninety-nine percent of the abnormal findings reported on a Pap smear are either completely benign or completely reversible and treatable. However, annual Pap smear testing remains important to ensure that any potentially serious problems are detected promptly.
Ritu Nayar, MD and David C. Wilbur, MD. The Pap test and Bethesda 2014. Article first published online: 1 MAY 2015. Cancer Cytopathology Volume 123, Issue 5, Pp. 271-281. Available online at http://onlinelibrary.wiley.com/doi/10.1002/cncy.21521/full through http://onlinelibrary.wiley.com. Accessed August 2015.
© 2017 American Association for Clinical Chemistry, republished from Lab Tests Online.*
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