Epstein-Barr Virus (EBV), Quantitative PCR

CPT: 87799
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Synonyms

  • EBV Viral Load
  • Epstein-Barr Virus (EBV), Quantitative, DNA by Real-time PCR

Expected Turnaround Time

2 - 4 days


Related Documents


Specimen Requirements


Specimen

Plasma (EDTA)


Volume

1 mL


Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Container

Plasma from a lavender-top tube. Ship in a screw-cap polypropylene frozen transport tube.


Collection

Collect specimen in lavender-top (EDTA) tube. Centrifuge specimen within 24 hours of collection. Remove plasma, transfer plasma to a screw-cap polypropylene transport tube, and freeze. Ship frozen (preferred). To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Freeze.


Stability Requirements

Temperature

Period

Room temperature

48 hours

Refrigerated

8 days

Frozen

6 months

Freeze/thaw cycles

Stable x4


Causes for Rejection

Quantity not sufficient for analysis; gross specimen contamination; specimen too old; leaking or broken tube


Test Details


Use

This assay is intended to be used for the quantitative detection of Epstein-Barr virus (EBV) DNA and as an aid in the diagnosis and management of EBV infections. This assay is traceable to the 1st WHO International Standard for Epstein Barr Virus for Nucleic Acid Amplification Techniques (NAT)-based assays.


Limitations

The quantitative range is 35.0 IU/mL to 100,000,000 IU/mL, and the analytical sensitivity is 18.8 IU/mL.


Methodology

Real-time polymerase chain reaction (PCR)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
138230 Epstein-Barr DNA Quant, PCR 43730-1 139811 EBV DNA, Quant PCR, Plasma IU/mL 43730-1
138230 Epstein-Barr DNA Quant, PCR 43730-1 139812 log10 EBV DNA,Plasma log10 IU/mL 47982-4
Reflex Table for EBV DNA, Quant PCR, Plasma
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 139815 Epstein-Barr DNA Quant, PCR 139813 EBV DNA, Quant PCR, Plasma IU/mL 43730-1
Reflex Table for EBV DNA, Quant PCR, Plasma
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 139815 Epstein-Barr DNA Quant, PCR 139814 log10 EBV DNA,Plasma log10 IU/mL 47982-4

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