<i>Mycoplasma/Ureaplasma</i>, Real-Time PCR
| Mycoplasma/Ureaplasma, Real-Time PCR | | | |
| Number | | 138778 |
| CPT | | 87801 |
| Synonyms | | Mycoplasma, DNA ; Mycoplasma, PCR ; Ureaplasma, DNA ; Ureaplasma, PCR |
Specimen | | Urogenital swab or urine |
| Volume | | One swab or 10 mL urine |
| Minimum Volume | | One swab or 1.0 mL urine |
| Container | | Universal transport medium - swab, sterile container - urine |
| Storage Instructions | | Refrigerate
Stability:
Swab: Room temperature or refrigerated up to 7 days
Urine: Refrigerated up to 7 days |
| Causes for Rejection | | Quantity not sufficient for analysis; gross contamination; specimen too old; leaking or broken tube |
| Use | | Detect the presence of Mycoplasma sp and Ureaplasma urealyticum DNA in clinical specimens |
| Methodology | | Polymerase chain reaction (PCR) amplification and real-time detection |
| References | | Stellrecht KA, Woron AM, Mishrik NG, et al, “Comparison of Multiplex PCR Assay With Culture for Detection of Genital Mycoplasmas,” J Clin Microbiol, 2004, 42(4):1528-33. Yoshida T, Maeda S, Deguchi T, et al, “Rapid Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum Organisms in Genitourinary Samples by PCR-Microtiter Plate Hybridization Assay,” J Clin Microbiol, 41(5):1850-5. |
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