Human Antimouse Antibodies
| Human Antimouse Antibodies | | | |
| Number | | 140657 |
| CPT | | 83520 |
| Synonyms | | HAMA |
| Specimen | | Serum |
| Volume | | 0.4 mL |
| Minimum Volume | | 0.2 mL (Note: This volume does not allow for repeat testing.) |
| Container | | Red-top tube or gel-barrier tube |
| Collection | | If a red-top tube is used, transfer separated serum to a plastic transport tube. |
| Storage Instructions | | Refrigerate |
| Causes for Rejection | | Nonserum specimen; gross lipemia |
| Reference Interval | | 0-188 ng/mL |
| Limitations | | This procedure may be considered by Medicare and other carriers as investigational and, therefore, may not be payable as a covered benefit for patients. |
| Methodology | | Enzyme immunoassay (EIA) |
| Additional Information | | The human antimouse antibodies (HAMA) direct ELISA test is intended for the determination of IgG subclass of isotypic HAMA in human serum. HAMA have been reported to give false-positive results in two-site immunometric assays that utilize murine (mouse) monoclonal IgG. HAMA reactivity has been detected in ~9% of a normal population without known exposure to murine IgG. Such responses may be due to polyclonal rheumatoid factors, heterophilic antibodies, dietary, or other exposure. Therapeutic use of murine monoclonal antibodies (IgG) or their fragments either unmodified or conjugated to drugs, toxins or radionucleotides may induce immune response directed against the same IgG and produce a significant level of HAMA in serum. Circulating levels of HAMA can bind to the injected IgG and reduce the efficacy of the antibody therapy. HAMA also can cause anaphylactic complications to subsequent administration of murine monoclonal IgG. |
| References | | Boscato LM and Stuart MC, “Heterophilic Antibodies: A Problem for All Immunoassays,” Clin Chem, 1988, 34(1):27-33. Dillman RO, Beauregard JC, Halpern SE, et al, “Toxicities and Side Effects Associated With Intravenous Infusions of Murine Monoclonal Antibodies,” J Biol Response Mod, 1986, 5(1):73-84. Kricka LJ, Schmerfeld-Pruss D, Senior M, et al, “Interference by Human Antimouse Antibody in Two Site Immunoassays,” Clin Chem, 1990, 36(6):892-4. |
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