Endomysial Antibody, IgA
| Endomysial Antibody, IgA | | | |
| Number | | 164996 |
| CPT | | 86255 |
| Specimen | | Serum |
| Volume | | 1 mL |
| Minimum Volume | | 0.3 mL (Note: This volume does not allow for repeat testing.) |
| Container | | Red-top tube or gel-barrier tube |
| Collection | | To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. |
| Storage Instructions | | Store at room temperature for up to 7 days prior to assay or for prolonged storage, keep undiluted at -20°C or below. |
| Causes for Rejection | | Lipemia; hemolysis; microbially-contaminated sera |
| Use | | Tissue transglutaminase is the autoantigen recognized by endomysial antibody in celiac disease.1 |
| Methodology | | Indirect fluorescent antibody (IFA) |
| Footnotes | | - Dieterich W, Ehnis T, Bauer M, et al, “Identification of Tissue Transglutaminase as the Autoantigen of Celiac Disease,” Nat Med, 1997, 3(7):797-801
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| References | | Biagi F, Pezzimenti D, Campanella J, et al, “Endomysial and Tissue Transglutaminase Antibodies in Coeliac Sera: A Comparison not Influenced by Previous Serological Testing,” Scand J Gastroenterol, 2001, 36(9):955-8. National Institute of Health Consensus Development Conference Statement on Celiac Disease, June 2004. |
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