Hemoglobin (Hgb) Solubility With Reflex to Hemoglobin Fractionation, Blood
| Hemoglobin (Hgb) Solubility With Reflex to Hemoglobin Fractionation, Blood | | | |
| Number | | 005330 |
| CPT | | 85660 |
| Related Information | | Hemoglobin (Hgb) Solubility |
| Synonyms | | Hb S ; Hemoglobin S ; Hgb Solubility ; Sickle Cell Preparation ; Sickle Cell Solubility Test ; Sickle Cell Test ; Sickledex™ ; Sickle Prep |
| Test Includes | | Hemoglobin (Hgb) solubility and automatic reflex to hemoglobin (Hgb) fractionation blood at additional charge, if positive. |
| Specimen | | Whole blood |
| Volume | | 1 mL |
| Minimum Volume | | 0.6 mL |
| Container | | Lavender-top (EDTA) tube; capillary puncture: three microhematocrit tubes. May also use blue-top (sodium citrate) tube, yellow-top (ACD) tube, or green-top (heparin) tube. |
| Storage Instructions | | Samples are stable when refrigerated (2°C to 8°C) for up to 2 weeks. |
Patient Preparation | | No preliminary fluid or food restriction is required. |
| Causes for Rejection | | Specimen other than whole blood received; clotted specimen |
| Reference Interval | | Negative |
| Use | | Qualitative determination of presence of hemoglobin S; detect sickling hemoglobins; evaluate hemolytic anemia, undiagnosed hereditary anemia with morphologic (sickle-like) abnormalities on peripheral blood smear |
| Limitations | | See Hemoglobin (Hgb) Solubility [005223] . |
| Methodology | | Sodium hydrosulfite reduction |
| Additional Information | | See Hemoglobin (Hgb) Solubility [005223] . |
| Footnotes | | - Miale JB, Laboratory Medicine: Hematology, 6th ed, St Louis, MO: Mosby-Year Book Inc, 1982, 624-5.
- Chehab FF and Kan YW, “Detection of Sickle Cell Anaemia Mutation by Colour DNA Amplification,” Lancet, 1990, 335(8680):15-7
|
|