Immunofixation (IFE), Serum and Protein Electrophoresis, Serum
| Immunofixation (IFE), Serum and Protein Electrophoresis, Serum | | | |
| Number | | 001495 |
CPT | | 82784 (x3) (IgG, IgA, IgM); 84165 (protein electrophoretic
fractionation and quantitation); 86334 (immunofixation
electrophoresis); 84155 |
| Related Information | | Immunofixation (IFE), Serum |
| Synonyms | | Globulins, Serum ; IEP ; IEP, Serum ; IFE, Serum and Protein Electrophoresis, Serum ; Immunoelectrophoresis for Myeloma Proteins ; Immunoelectrophoresis, Serum ; Immunofixation, Serum ; Protein IEP ; Serum IFE ; Serum Immunofixation |
| Test Includes | | Quantitation of immunoglobulins IgA, IgG, and IgM; IFE interpretation of heavy- and light-chain patterns; serum protein electrophoresis |
| Special Instructions | | State patient's age and clinical diagnosis on the request form. |
| Specimen | | Serum |
| Volume | | 3 mL |
| Minimum Volume | | 2 mL |
| Container | | Red-top tube or gel-barrier tube |
| Collection | | Separate serum from cells. |
| Storage Instructions | | Refrigerate |
| Patient Preparation | | Patient should be fasting. |
| Causes for Rejection | | Gross hemolysis; gross lipemia |
| Reference Interval | | Normal pattern |
| Use | | Detect and identify monoclonal immunoglobulin gammopathies |
| Methodology | | Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); protein electrophoresis on agarose media; turbidimetric quantitation of IgA, IgG, and IgM |
| Additional Information | | Diagnose myeloma, macroglobulinemia of Waldenström. Immunofixation is used to evaluate monoclonal gammopathy (M protein) found on serum protein electrophoresis; evaluate amyloidosis. Has application to the evaluation of lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general. Of use in the diagnosis and characterization of immune deficient and dysgammaglobulinemic states. |
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