17-Hydroxycorticosteroids and 17-Ketosteroids
| 17-Hydroxycorticosteroids and 17-Ketosteroids | | | |
| Number | | 020966 |
| CPT | | 83491; 83586 |
| Related Information | | Urine Testing: Preservative Quick Reference Chart |
| Synonyms | | 17-Hydroxycorticosteroids ; 17-Ketosteroids ; 17-Ketosteroids and Porter-Silber Chromogens ; 17-KS and 17-OHCS ; 17-OHCS and 17-KS ; Porter-Silber Chromogens and 17-Ketosteroids |
| Test Includes | | 17-hydroxycorticosteroids, total, urine; 17-ketosteroids, total, urine |
| Special Instructions | | State patient's sex and total 24-hour urine volume on the request form. |
| Specimen | | Urine (24-hour) |
| Volume | | 100 mL aliquot |
| Minimum Volume | | 40 mL aliquot |
| Container | | Plastic urine container with 1 g boric acid per liter of urine. 30 mL 6N HCl may also be used as a preservative. |
| Storage Instructions | | Refrigerate |
| Patient Preparation | | If possible, all drugs should be withheld for 72 hours prior to and during collection of urine. |
| Causes for Rejection | | Preservative not added |
| Use | | Adrenal function test; useful in the evaluation of glucocorticoid production and neuroendocrine function; useful in the evaluation of androgenic adrenal and testicular function in normal males and primarily adrenal androgenic secretion in normal females. Urinary metabolites of glucocorticoids can be measured by 17-ketogenic steroids and 17-hydroxycorticosteroids. More metabolites are measured by the former.1 |
| Methodology | | 17-OHCS: Porter-Silber; 17-KS: Drekter-Zimmerman modified reaction |
| Additional Information | | The Porter-Silber color reaction detects steroids with a dihydroxyacetone group at carbon 17, including major glucocorticoid metabolites. By pretreating with a strong reducing agent one increases the number of metabolites detected (ie, 17-ketogenic steroids). Either 17-hydroxysteroid or 17-ketogenic steroid measurements can be used as an estimate of adrenal steroid production, either as baseline values or in stimulation or suppression tests. Deoxycorticosterone (DOC), corticosterone (compound B) and aldosterone lack a 17-hydroxyl group and are not measured in 17-KG and 17-OHCS procedures.1 |
| Footnotes | | - Speroff L, Glass RH, and Kase NG, Clinical Gynecologic Endocrinology and Infertility, 4th ed, Baltimore, MD: Williams & Wilkins, 1989
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