5′ Nucleotidase
| 5′ Nucleotidase | | | |
| Number | | 001701 |
| CPT | | 83915 |
| Specimen | | Serum |
| Volume | | 2 mL |
| Minimum Volume | | 0.2 mL |
| Container | | Red-top tube or gel-barrier tube |
| Collection | | To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. |
| Storage Instructions | | Serum may be stored up to 1 week refrigerated at 2°C to 8°C or 2 weeks frozen at -20°C. |
| Causes for Rejection | | Hemolysis; plasma specimen |
| Reference Interval | | 0-10 IU/L |
| Use | | 5′ nucleotidase is used to investigate the origin of increased serum alkaline phosphatase. It is a liver-related enzyme used to work up cholestatic/biliary obstruction. It parallels the increases of alkaline phosphatase and leucine aminopeptidase in hepatobiliary diseases, but is not usually elevated in skeletal disorders such as Paget disease of bone. It is increased with metastatic neoplasia in liver and in primary biliary cirrhosis, biliary obstruction from calculi and tumor. |
| Methodology | | Kinetic - 340 nm |
|