Celiac Disease Antibody Screen

Celiac Disease Antibody Screen

Number
	334971
CPT
	82784; 83516 (x2)
Synonyms
	Celiac ; Gliadin ; Deamidated Gliadin Antibodies (DGP) ; tissue transglutaminase (tTG) Antibodies
Test Includes
	Deamidated gliadin IgA; tissue transglutaminase IgA; and serum IgA quantitation
Specimen
	Serum
Volume
	3 mL
Minimum Volume
	1 mL
Container
	Red-top tube or gel-barrier tube
Collection
	To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
	Ship at room temperature, store refrigerated for up to 48 hours and then freeze at -20°C for longer storage
Causes for Rejection
	Hemolysis; lipemia; gross bacterial contamination
Use
	Aid in the diagnosis of gluten-sensitive enteropathies.
Methodology
	Enzyme immunoassay (EIA); indirect immunofluorescence
Additional Information
	Celiac disease is a gluten enteropathy occurring in both children and adults. The disease is probably underdiagnosed in that it may affect as much as 1% of the population in the United States. The condition is characterized by a sensitivity to gluten (found in wheat, barley, and rye) that causes inflammation and atrophy of the villi of the small intestine, malabsorption, etc. This sensitivity to gluten may also be seen in dermatitis herpetiformis. Strict avoidance of gluten in the diet will control disease activity, and antibodies to serum markers will disappear with time.
References
	NIH Consensus and State-of-the-Science Statement on Celiac Disease. NIH Consens State Sci Statements. 2004; 21:1-22. Agardh D. Antibodies against synthetic deamidated gliadin peptides and tissue transglutaminase for the identification of childhood celiac disease. Clin Gastroenterol Hepatol. 2007 Nov; 5(11):1276-1281. Green PH, Cellier C. Celiac disease. N Engl J Med. 2007 Oct 25; 357(17):1731-1743. Hill ID, Dirks MH, Liptak GS, et al. Guideline for the diagnosis and treatment of celiac disease in children: Recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition. J Pediatr Gastroenterol Nutr. 2005 Jan; 40(1):1-19. Kaukinen K, Collin P, Laurila K, Kaartinen T, Partanen J, Mäki M. Resurrection of gliadin antibodies in coeliac disease. Deamidated gliadin peptide antibody test provides additional diagnostic benefit. Scand J Gastroenterol. 2007 Dec; 42(12):1428-1433. Mothes T. Deamidated gliadin peptides as targets for celiac disease-specific antibodies. Adv Clin Chem. 2007; 44:35-63. Rashtak S, Ettore MW, Homburger HA, Murray JA. Comparative usefulness of deamidated gliadin antibodies in the diagnosis of celiac disease. Clin Gastroenterol Hepatol. 2008 Apr; 6(4):426-432; quiz 370. Schwertz E, Kahlenberg F, Sack U, et al. Serologic assay based on gliadin-related nonapeptides as a highly sensitive and specific diagnostic aid in celiac disease. Clin Chem. 2004 Dec; 50(12):2370-2375. Sugai E, Vázguez H, Nachman F, et al. Accuracy of testing for antibodies to synthetic gliadin-related peptides in celiac disease. Clin Gastroenterol Hepatol. 2006 Sep; 4(9):1112-1117.