Glutathione S-transferase Gene (GSTP1, pi-class)
Methylation Assay
Glutathione S-transferase Gene (GSTP1, pi-class)
Methylation Assay | | | |
| Number | | 489320 |
| CPT | | 83891; 83896 (x4); 83898 (x2); 83900; 83907; 83912 |
| Synonyms | | GSTP1 |
| Special Instructions | | Please provide a copy of the pathology report. Please
direct any questions regarding this test to Customer
Service at 800-533-0567. |
| Specimen | | Formalin-fixed, paraffin-embedded tissue (FFPE) |
| Volume | | Formalin-fixed, paraffin-embedded tissue block |
| Minimum Volume | | Five 5μm tissue sections |
| Container | | Paraffin block |
| Storage Instructions | | Maintain specimen at room temperature. |
| Causes for Rejection | | Paraffin block containing insufficient tissue. |
| Use | | Prostate cancer is the most common cancer in men and the
second leading cause of cancer-related deaths in the United
States. Due to the reportedly high false negative rate of
initial biopsy results after an elevated PSA level, new
approaches for improved detection in prostate cancer are
needed. Several studies have shown that hyper-methylation
of the promoter regions of the GSTP1 and APC genes occur at
a significantly higher frequency in prostate cancer samples
than in benign conditions of the prostate
gland.1,2 For that
reason, gene methylation assays may be used as an adjunct
to histopathology for patients where prostate disease is
considered. It has been shown that the methylation levels
for genes, such as GSTP1, may increase with patient age.
Therefore, age should be considered along with other
clinical features in the interpretation of test
results.3 |
| Limitations | | Preparation of DNA from tissue samples is dependent upon
the quality of the provided specimen. Inadequate DNA
extraction may occur in a significant number of
paraffin-embedded samples. |
| Methodology | | Quantitative methylation specific PCR |
| Footnotes | |
- Li LC, Carrol PR, Dahiga R. Epigenetic
Changes in Prostate Cancer: Implications for Diagnosis and
Treatment. Journal of the National Cancer Institute
97: 103; 2005.
- Bastian PJ, Ellinger J, Wellman A. et al.
Diagnostic and Prognostic Information in Prostate Cancer
with the Help of a Small Set of Hypermethylated Gene Loci.
Clinical Cancer Research 11: 4097; 2005.
- Kwabe-Addo B, Chung W, Shen L. et al. Age
Related DNA Methylation Changes in Normal Human Prostate
Tissue. Clinical Cancer Research 13: 3796;
2007.
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