Hepatitis B Virus (HBV) QuantaSure By Real-time PCR With
Reflex to HBV Drug Resistance, INNO-LiPA
| Hepatitis B Virus (HBV) QuantaSure By Real-time PCR With
Reflex to HBV Drug Resistance, INNO-LiPA | | | |
| Number | | 551570 |
CPT | | 87517 |
| Synonyms | | HBV QuantaSure, HBV Quantitation, TaqMan HBV QuantaSure |
| Special Instructions | | A separate frozen specimen is required for each frozen test
ordered. If reflex test is performed, additional
charges/CPT code(s) may apply. |
| Specimen | | Serum or plasma, frozen |
| Volume | | 2 mL |
| Container | | Plasma Preparation Tube PPT™ or a screw-cap
polyproplyene frozen transport tube. |
| Collection | | Collect whole blood in PPT™, red top tube,
gel-barrier tube, yellow-top (ACD) tube, or lavender-top
(EDTA) tube. Do not use green-top (heparin) tubes.
Centrifuge specimen within four hours of collection, remove
plasma, and transfer specimen to a plastic screw-cap
transport tube and freeze. Ship frozen on dry ice. If
PPT™ is used, do not transfer plasma; centrifuge
specimen in a swinging bucket rotor centrifuge and ship
frozen on dry ice. To avoid delays in turnaround time when
requesting multiple tests on frozen samples, please submit
separate frozen specimens for each test requested. |
| Storage Instructions | | Freeze |
| Causes for Rejection | | Hemolysis; green-top (heparin) tube; specimen not frozen;
specimen received in "pop-top" or "snap-cap" tube;
PPT™ not centrifuged |
| Use | | Quantitation of HBV DNA in serum or plasma with reflex to
HBV Drug Resistance, INNO-LiPA if greater than 1,000
copies/mL. |
| Limitations | | The HBV QuantaSure assay has a quantitative range of 100 to
5,000,000,000 copies/mL. |
| Methodology | | TaqMan™ polymerase chain reaction (PCR) amplification
and detection |
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