Epstein-Barr Virus (EBV), DNA Probe
| Epstein-Barr Virus (EBV), DNA Probe | | | |
| Number | | 550913 |
| CPT | | 88365 |
| Synonyms | | EBV in situ Hybridization |
| Test Includes | | DNA probe hybridization for EBV in formalin-fixed, paraffin-embedded tissue |
| Special Instructions | | Please direct any questions regarding this test to Oncology Customer Service at 800-533-0567, ext 4060. |
| Specimen | | Formalin-fixed, paraffin-embedded tissue biopsy. Tissue biopsies should be fixed in 10% neutral-buffered formalin and embedded in paraffin within 72 hours. Do not submit frozen tissue or substitute other fixatives. For tissues submitted in formalin, please provide the appropriate gross/microscopic description indicating the number, type, and sites of the tissue submitted. |
| Container | | Submit paraffin block in crushproof container. Submit fresh tissue in a sterile container in 10% neutral-buffered formalin. |
| Collection | | Tissue biopsies should be fixed in 10% neutral buffered formalin and embedded in paraffin within 72 hours. Do not submit frozen tissue or substitute other fixatives. For tissues submitted in formalin, please provide appropriate gross/microscopic description indicating the number, type, and site of the tissue(s) submitted. |
| Causes for Rejection | | Insufficient tissue; improper fixation; inappropriate fixative |
| Use | | Detect EBV DNA sequences in tissue sections; adjunct to histopathology |
| Limitations | | Failure to detect target DNA may result from several factors such as improper sampling, handling, and/or fixative of the specimen, or target DNA below the sensitivity limits of the assay. Therefore, lack of staining does not necessarily imply the absence of target DNA. This procedure may be considered by Medicare and other carriers as investigational and, therefore, may not be payable as a covered benefit for patients. |
| Methodology | | In situ hybridization |
| References | | Berg LC, Copenhaver CM, Morrison VA, et al, “B-Cell Lymphoproliferative Disorders in Solid-Organ Transplant Patients: Detection of Epstein-Barr Virus by in situ Hybridization,” Hum Pathol, 1992, 23(2):159-63. De Souza YG, Freese VK, Greenspan D, et al, “Diagnosis of Epstein-Barr Virus Infection in Hairy Leukoplakia by Using Nucleic Acid Hybridization and Noninvasive Techniques,” J Clin Microbiol, 1990, 28(12):2775-8. Hamilton-Dutoit SJ, Delecluse HJ, Raphael M, et al, “Detection of Epstein-Barr Virus Genomes in AIDS Related Lymphomas: Sensitivity and Specificity of in situ Hybridization Compared With Southern Blotting,” J Clin Pathol, 1991, 44(8):676-80. |
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