Myelodysplastic Syndrome, Fluorescence <i>in situ</i> Hybridization (FISH)
| Myelodysplastic Syndrome, Fluorescence in situ Hybridization (FISH) | | | |
| Number | | 510599 |
| CPT | | 88271 (x4); 88275; 88291 |
| Related Information | | Leukemia / Lymphoma Management Test Reference Chart |
| Synonyms | | MDS ; Myelodysplastic Syndrome FISH |
| Special Instructions | | Indicate pertinent clinical diagnosis and previous cytogenetic studies on the request form. This test is for use on blood samples when no results are available from chromosome analysis and should not be ordered in conjunction with the preferred chromosome analysis. |
| Specimen | | Blood, bone marrow, fixed-cell pellet from a cytogenetic analysis, or slides prepared from a cytogenetic analysis |
| Volume | | Pediatrics: 3-5 mL blood, adults: 10 mL blood; 1-3 mL bone marrow, four slides, one fixed-cell pellet |
| Minimum Volume | | 1 mL blood (adult/pediatric), 1 mL bone marrow, or two slides |
Container | | Green-top (heparin) tube; pediatric Vacutainer® is optimal
or lavender-top (EDTA) tube |
| Storage Instructions | | Maintain specimen at room temperature. |
| Causes for Rejection | | Broken Vacutainer®; broken slides |
| Use | | Diagnostic test for myelodysplastic syndrome. Principle use is for interphase analysis of cases with no or low mitotic activity in cytogenetic analysis or interphase analysis from blood in cases of inaspirable bone marrow. Detection rate is ~80% of clones detected in cytogenetic analysis. |
| Methodology | | Fluorescence in situ hybridization (FISH) for 5q, 7q, 8, and 20q |
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